An analysis of the polymorphisms in a gene for being involved in drought tolerance in maize
An analysis of the polymorphisms in a gene for being involved in drought tolerance in maize
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DOI：
10.1007/s10709-011-9568-y
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发表时间：
2011-04-01
期刊：
影响因子：
1.5
通讯作者：
Zhang, Shihuang
中科院分区：
文献类型：
--
作者：
Li, Liang;Hao, Zhuanfang;Zhang, Shihuang
植物蔗糖酶催化蔗糖转化为葡萄糖和果糖，它们是各种胁迫耐受过程的独特信号，包括缺水条件下激素的生物合成和检测。在蔗糖酶基因家族中，编码植物酸溶性蔗糖酶的候选基因ivr2在耐旱性中发挥着至关重要的作用。在本研究中，基于GenBank文库的部分ivr2基因序列，获得了包含三个外显子和两个内含子的ivr2推定基因组序列，并利用生物信息学和统计学进行了遗传分析。使用五个嵌套引物对获得了 106 个玉米自交系的 ivr2 基因组序列数据。进一步分析发现，检测到的多态性位点主要位于外显子1、内含子1和外显子2区域；在该 ivr2 基因座上鉴定出高连锁不平衡水平和低核苷酸多样性。关联作图结合了基因型和表型数据，在两年的实验中，在水分充足和缺水条件下，总共 48 个关联显示出对谷物产量及其组成部分的变化有很高的贡献。这表明ivr2的功能多态性可能与玉米的耐旱性有关。这为耐旱育种计划中优良等位基因的有效标记辅助选择提供了参考信息。
Plant invertases catalyze the conversion of sucrose to glucose and fructose, which are distinct signals of widely varied stress-tolerance processes, including the biosynthesis and detection of hormones under water deficit. In the invertase gene family, the candidate gene ivr2 encoding plant acid-soluble invertase plays a vital role in drought tolerance. In this study, a putative genomic sequence of ivr2 including three exons and two introns was acquired and genetically analyzed using bioinformatics and statistics, based on a partial ivr2 gene sequence of the GenBank library. The ivr2 genomic sequence data from 106 maize inbred lines were obtained using five nested primer pairs. Further analysis showed that the detected polymorphic sites were mainly located in exon-1, intron-1 and exon-2 regions; High linkage disequilibrium level and low nucleotide diversity were identified at this ivr2 locus. Association mapping combined the genotypic and phenotypic data, and a total of 48 associations showed high contributions to the variations in grain yield and its components under well-watered and water-stressed conditions over 2 years of experiments. This suggested that functional polymorphisms in ivr2 were possibly associated with maize drought tolerance. This provides reference information for efficient marker-assisted selection of superior alleles in drought tolerance breeding programs.