The presence of endometrial cells in the peritoneal cavity enhances monocyte recruitment and induces inflammatory cytokines in mice: Implications for endometriosis
The presence of endometrial cells in the peritoneal cavity enhances monocyte recruitment and induces inflammatory cytokines in mice: Implications for endometriosis
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DOI：
10.1016/j.fertnstert.2004.04.040
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发表时间：
2004-10-01
影响因子：
6.7
通讯作者：
Parthasarathy, S
中科院分区：
文献类型：
--
作者：
Cao, X;Yang, DZ;Parthasarathy, S
目的：通过子宫内膜细胞的存在和间皮的作用来确定腹膜腔内的炎症反应。设计：小鼠体内研究。环境：大学研究实验室。动物：雌性瑞士韦氏鼠，8 ~ 10周龄。干预：腹腔注射小鼠子宫内膜上皮细胞和基质细胞。给药后4 ~ 72小时收集腹腔灌洗及间皮。主要观察指标：测定腹腔巨噬细胞数量、单核细胞趋化蛋白-1 （MCP-1/JE）、白细胞介素1 α (il -1 α)和白细胞介素6 （IL-6）的产生和基因表达。结果：子宫内膜细胞腹腔注射增加了受体小鼠腹膜巨噬细胞的数量，MCP-1、il -1 α和IL-6的产生，以及间皮MCP-1/JE、il -1 α和IL-6基因的表达。结论：这些结果表明，月经逆行可能是子宫内膜异位症患者腹腔炎症介质增加的原因。间皮除了为子宫内膜细胞提供附着层外，还可能在子宫内膜异位症中发挥积极作用。(C) 2004年，美国生殖医学学会。
Objective: To determine the inflammatory response in the peritoneal cavity by the presence of endometrial cells and the role of the mesothelium.Design: In vivo study using mice.Setting: University research laboratory.Animal(s): Female Swiss Webster mice, 8 to 10 weeks old.Intervention(s): Homogenous mouse endometrial epithelial and stromal cells were injected intraperitoneally. Peritoneal lavage and mesothelium were collected 4 to 72 hours after the administration.Main Outcome Measure(s): We determined the number of peritoneal macrophages, and the production and gene expression of monocyte chemotactic protein-1 (MCP-1/JE), interleukin 1alpha (IL-1alpha), and interleukin 6 (IL-6).Result(s): The intraperitoneal administration of endometrial cells increased the number of peritoneal macrophages, production of MCP-1, IL-1alpha, and IL-6, and expression of mesothelial MCP-1/JE, IL-1alpha, and IL-6 genes in recipient mice.Conclusion(s): These results suggest that retrograde menstruation could account for the increased presence of inflammatory mediators in the peritoneal cavity of women with endometriosis. The mesothelium could play an active role in endometriosis in addition to providing an attachment stratum for the endometrial cells. (C) 2004 by American Society for Reproductive Medicine.