Interaction of clinical isolates of Streptococcus pneumoniae with human complement factor H.
Interaction of clinical isolates of Streptococcus pneumoniae with human complement factor H.
复制标题
肺炎链球菌临床分离株与人补体因子 H 的相互作用。
DOI：
10.1111/j.1574-6968.2006.00439.x
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发表时间：
2006
影响因子：
2.1
通讯作者：
McDaniel,LarryS
中科院分区：
文献类型：
--
作者：
Quin,LisaR;Onwubiko,Chinwendu;Carmicle,Stephanie;McDaniel,LarryS
PspC将补体因子H（FH）募集到肺炎球菌表面。虽然在感染过程中存在差异表达的PspC，但由于PspC之间的变异性，在活的肺炎球菌表面上检测PspC是困难的。我们分析了FH结合，以检测来自不同病理来源的肺炎球菌分离株表面上的PspC表达。使用流式细胞术，我们研究了FH结合89低传代临床分离株分类的疾病表现（全身，粘膜，或携带）。携带分离株招募显着更多的FH比系统或粘膜分离株的表面，这种结合是独立的荚膜血清型。
PspC recruits complement factor H (FH) to the pneumococcal surface. While there is differential expression ofpspCduring infection, detection of PspC on the surface of viable pneumococci is difficult due to variability among PspCs. We analyzed FH binding to detect PspC expression on the surface of pneumococcal isolates from different pathological sources. Using flow cytometry, we investigated FH-binding to 89 low-passage clinical isolates classified by disease manifestation (systemic, mucosal, or carriage). Carriage isolates recruited significantly more FH to their surfaces than either systemic or mucosal isolates, and this binding was independent of capsular serotype.