Discrimination and genetic diversity of Wheat dwarf virus in the Czech Republic
Discrimination and genetic diversity of Wheat dwarf virus in the Czech Republic
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DOI：
10.1007/s11262-009-0352-3
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发表时间：
2009-06-01
期刊：
影响因子：
1.6
通讯作者：
Cervena, G.
中科院分区：
文献类型：
--
作者：
Kundu, J. K.;Gadiou, S.;Cervena, G.
感染谷物的mastrevirus是最普遍存在的病毒之一，在过去十年中在捷克共和国造成了巨大的产量损失。在小麦和大麦作物的田间样本中证实存在两株小麦矮病毒（WDV），其中一株是小麦适应型，另一株是大麦适应型。采用PCR-RFLP和基于测序的方法进行病毒分型。捷克大麦菌株的WDV分离株比小麦菌株的分离株更具变异性，将它们分为两个分支，一个代表不同分离株的池。RFLP分析证实捷克分离株分离为两个菌株，并强调了一种快速诊断小麦和大麦菌株的有效方法。此外，RFLP和序列分析表明，大麦菌株局限于大麦寄主，而小麦菌株存在于小麦和大麦植株中。
Cereal-infecting Mastreviruses are one of the most ubiquitous of viruses, having caused huge yield losses during the last decade in the Czech Republic. The presence of two strains of Wheat dwarf virus (WDV), one of which is wheat adapted and one barley adapted, have been confirmed from among field samples of wheat and barley plants. The virus typing was conducted by both PCR-RFLP and sequencing-based methods. The Czech WDV isolates of the barley strain are more variable than the isolates of the wheat strain, separating them into two clades, one representing a pool of divergent isolates. The RFLP analysis confirmed the separation of the Czech isolates into two strains and highlights a powerful method for the rapid diagnosis of both the wheat and barley strains. Additionally, both RFLP and sequence analysis have shown that the barley strain is restricted to the barley host, while the wheat strain is present in both wheat and barley plants.