The 5-HT1A serotonin receptor is located on calbindin- and parvalbumin-containing neurons in the rat brain
The 5-HT1A serotonin receptor is located on calbindin- and parvalbumin-containing neurons in the rat brain
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DOI：
10.1016/s0006-8993(02)03727-7
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发表时间：
2003-01-03
期刊：
影响因子：
2.9
通讯作者：
Knudsen, GM
中科院分区：
文献类型：
--
作者：
Aznar, S;Qian, ZX;Knudsen, GM
5-HT1A 受体是一种特征明确的血清素受体，在许多中枢神经功能中发挥作用，并已知与抑郁症和其他精神障碍有关。原位杂交、免疫细胞化学和结合研究表明，5-HT1A受体在大鼠大脑中广泛分布，在边缘系统中密度特别高。然而，受体在不同神经元亚型中的定位对于理解其在神经元回路中的作用可能很重要，但目前尚不清楚。在这项研究中，我们通过免疫细胞化学双标记技术表明，5-HT1A 受体存在于锥体细胞和主细胞以及含有钙结合蛋白和小清蛋白的神经元上，它们通常定义了两种不同的中间神经元亚型。此外，半定量分析表明，不同神经元类型中受体的分布因大脑区域而异。在皮层、海马体、下丘脑和杏仁核中，该受体位于主细胞以及含有钙结合蛋白和小白蛋白的神经元上。在隔膜和丘脑中，受体主要存在于含有钙结合蛋白和小清蛋白的细胞上。特别是在内侧隔膜和丘脑网状核中，受体与小白蛋白阳性神经元高度共定位。这些结果表明 5-HT1A 受体在调节不同大脑区域的神经元回路中具有不同的功能，这可能取决于受体主要位于的神经元类型。 (C) 2002 Elsevier Science B.V. 保留所有权利。
The 5-HT1A receptor is a well-characterized serotonin receptor playing a role in many central nervous functions and known to be involved in depression and other mental disorders. In situ hybridization, immunocytochemical, and binding studies have shown that the 5-HT1A receptor is widely distributed in the rat brain, with a particularly high density in the limbic system. The receptor's localization in the different neuronal subtypes, which may be of importance for understanding its role in neuronal circuitries, is, however, unknown. In this study we show by immunocytochemical double-labeling techniques, that the 5-HT1A receptor is present on both pyramidal and principal cells, and calbindin- and parvalbumin-containing neurons, which generally define two different subtypes of interneurons. Moreover, semiquantitative analysis showed that the receptor's distribution in the different neuronal types varies between brain areas. In cortex, hippocampus, hypothalamus, and amygdala the receptor was located on both principal cells and calbindin- and parvalbumin-containing neurons. In septum and thalamus, the receptor was mostly present on calbindin- and parvalbumin-containing cells. Especially in the medial septum and thalamic reticular nucleus, the receptor highly colocalized with parvalbumin-positive neurons. These results suggest a diverse function of the 5-HT1A receptor in modulating neuronal circuitry in different brain areas, that may depend on the type of neuron the receptor is predominantly located on. (C) 2002 Elsevier Science B.V. All rights reserved.