DNA cleavage study using copper (II)-GlyAibHis: a tripeptide complex based on ATCUN peptide motifs.
DNA cleavage study using copper (II)-GlyAibHis: a tripeptide complex based on ATCUN peptide motifs.
复制标题
使用铜 (II)-GlyAibHis 进行 DNA 切割研究：基于 ATCUN 肽基序的三肽复合物。
DOI：
10.2174/092986608783330378
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发表时间：
2008
影响因子：
1.6
通讯作者：
U. Singh
中科院分区：
文献类型：
--
作者：
R. Singh;N. Sharma;R. Prasad;U. Singh
新型化学核酸酶由于其在生物技术中的广泛应用和作为治疗剂的广泛应用而引起人们极大的兴趣。ATCUN（氨基末端Cu（II）和Ni（II）结合）是天然存在于血清白蛋白中的肽基序。合成了具有非天然氨基酸Aib（α-氨基异丁酸）的类似肽基序（GlyAibHis），并通过ESI-MS和分光光度法研究其Cu（II）配合物。这种复合物对DNA切割的反应性进行了研究。Cu（II）-GlyAibHis仅在抗坏血酸盐等温和氧化剂存在下通过氧化机制而不是水解机制显示DNA切割，并遵循假一级动力学（K obs = 0.085 min（-1））。pNPP的水解进一步支持非水解机制，其遵循伪一级动力学（Kobs = 1.98 × 10（-2）min（-1）），没有pH影响。
Development of new chemical nucleases is a matter of great interest because of their extensive use in biotechnology and as therapeutic agents. The ATCUN (amino terminal Cu(II) and Ni(II) binding) is a peptide motif that occurs naturally in the serum albumins. The similar peptide motif (GlyAibHis) having unnatural amino acid Aib (alpha-aminoisobutyric acid) was synthesized and its Cu(II) complex was characterized by ESI-MS and spectrophotometry studies. The reactivity of this complex toward DNA cleavage has been investigated. Cu(II)-GlyAibHis shows the DNA cleavage only in presence of mild oxidizing agents like ascorbate by an oxidative mechanism rather than hydrolytic and follows the pseudo first order kinetics (K obs = 0.085 min(-1)). The non-hydrolytic mechanism was further supported by the hydrolysis of pNPP which followed the pseudo first order kinetics (K obs = 1.98 x 10(-2) min(-1)) having no pH effect.