Electrochemical Aflatoxin B1 immunosensor based on the use of graphene quantum dots and gold nanoparticles
Electrochemical Aflatoxin B1 immunosensor based on the use of graphene quantum dots and gold nanoparticles
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DOI：
10.1007/s00604-019-3701-5
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发表时间：
2019-08-01
影响因子：
5.7
通讯作者：
Sumana, Gajjala
中科院分区：
文献类型：
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作者：
Bhardwaj, Hema;Pandey, Manoj Kumar;Sumana, Gajjala
描述了一种用于黄曲霉毒素B1（AFB 1）的电化学免疫传感器，该传感器使用由石墨烯量子点（GQD）和金纳米颗粒（Au NPs）制备的复合物。通过使用2-氨基苯硫酚（ATP）作为接头获得GQD-AuNP缀合物，其中GQD的羧基基团通过结合到AuNP的硫醇的缀合而结合到交联剂的氨基基团。为了评估GQD-AuNP复合物的缀合，应用傅里叶变换红外光谱（FT-IR）和透射电子显微镜（TEM）。将复合物置于氧化铟锡（ITO）电极上，然后用抗AFB 1的抗体修饰。该传感器以铁氰化物为探针，在0.1 ~ 3.0ngmL（-1）AFB_1浓度范围内工作，特异性强，重现性好，稳定性好。将该传感器应用于玉米样品的分析。可以想象，该方法可以用于通过使用其各自的抗体来感测其他真菌毒素。
Electrochemical immunosensor for aflatoxin B1 (AFB1) is described that uses a composite prepared from graphene quantum dots (GQDs) and gold nanoparticles (Au NPs). The GQD-AuNP conjugate was obtained by using 2-aminothiophenol (ATP) as a linker where the carboxy groups of GQD bind to the amino groups of crosslinker via conjugation of thiol binding to the AuNP. To evaluate the conjugation of the GQD-AuNP composite, Fourier transform infrared spectroscopy (FT-IR), and transmission electron microscopy (TEM) was applied. The composite was placed on an indium tin oxide (ITO) electrode and then modified with an antibody against AFB1. By using hexacyanoferrate as the electrochemical probe, the sensor works in the 0.1 to 3.0ngmL(-1) AFB1 concentration range, is highly specific, has good reproducibility and acceptable stability. The biosensor was applied to the analysis of (spiked) maize samples. Conceivably, the method can be utilized to sense other mycotoxins by using their respective antibodies.