Mutational analysis of the adcCBA genes in Streptococcus gordonii biofilm formation.
Mutational analysis of the adcCBA genes in Streptococcus gordonii biofilm formation.
复制标题
戈登链球菌生物膜形成中 adcCBA 基因的突变分析。
DOI：
10.1111/j.1399-302x.2004.00205.x
复制
发表时间：
2005
期刊：
影响因子：
--
通讯作者：
Ganeshkumar,N
中科院分区：
文献类型：
--
作者：
Mitrakul,K;Loo,CY;Gyurko,C;Hughes,CV;Ganeshkumar,N
戈登链球菌是一种主要的定居者，是先驱生物膜财团的一部分，启动牙齿表面的牙菌斑发展。将Tn 917-lactransposon插入到theadcR基因中产生了生物膜缺陷型。戈登链球菌adcR是一种调控基因，是操纵子（cR）的一部分，包括其他三个基因adcCBA。 AdcC含有一个推定的三磷酸腺苷的共有结合位点，AdcB是一种推定的疏水膜蛋白，AdcA是一种推定的脂蛋白通透酶。通过插入失活三个adcCBA基因中的每一个构建突变体，并检测它们对生物膜形成的影响。TheadcC：：specRandadcB：：specRmutations显示出生物膜缺陷型表型，而theadcA：：specRmutant在静态聚苯乙烯微量滴定板生物膜测定中为生物膜阳性。所有三种突变体在流动池系统中形成不良的生物膜，并且是感受态缺陷的，这表明theadcoperon在戈登链球菌生物膜形成和感受态中起重要作用。
Streptococcus gordonii,a primary colonizer, is part of the pioneer biofilm consortium that initiates dental plaque development on tooth surfaces. An insertion of Tn917‐lactransposon into theadcRgene produced a biofilm‐defective phenotype.S. gordonii adcRis a regulatory gene and is part of an operon (adc) that includes three other genes,adcCBA. AdcC contains a putative consensus‐binding site for adenosine triphosphate, AdcB is a putative hydrophobic membrane protein, and AdcA is a putative lipoprotein permease. Mutants were constructed by insertional inactivation in each of the threeadcCBAgenes and their effects on biofilm formation examined. TheadcC::specRandadcB::specRmutations displayed a biofilm‐defective phenotype, whereas theadcA::specRmutant was biofilm‐positive in a static polystyrene microtiter plate biofilm assay. All three mutants formed poor biofilms in a flow‐cell system and were competence‐defective, suggesting theadcoperon plays an important role inS. gordoniibiofilm formation and competence.