Mutation of gdpS gene induces a viable but non-culturable state in Staphylococcus epidermidis and changes in the global transcriptional profile.
Mutation of gdpS gene induces a viable but non-culturable state in Staphylococcus epidermidis and changes in the global transcriptional profile.
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gdpS基因突变诱导表皮葡萄球菌存活但不可培养状态及整体转录谱的变化
DOI：
10.1186/s12866-022-02708-6
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发表时间：
2022-12-01
期刊：
影响因子：
4.2
通讯作者：
Wu, Yang
中科院分区：
文献类型：
--
作者：
Zhu, Tao;Wang, Wei;Wang, Han;Zhao, Yanfeng;Qu, Di;Wu, Yang
在葡萄球菌的基因组中，只有gdpS基因编码保守的GGDEF结构域，这是双鸟苷酸环化酶的特征。在我们以前的研究中，我们已经证明，gdpS基因可以调节生物膜的形成，通过积极调节伊卡操纵子在表皮葡萄球菌的表达。此外，这种调节似乎不依赖于c-di-GMP信号通路和该基因的蛋白质编码功能。因此，gdpS基因的生物学功能还有待进一步研究。在本研究中，观察到gdpS基因突变诱导S。表皮细胞在用甘油冷冻保存后进入假定的可存活但不可培养的状态（VBNC）。类似地，当从液体培养基转移到固体培养基时，gdpS突变株也表现出VBNC状态。与野生型菌株相比，gdpS突变株在4℃条件下的自溶速度更快，表明其对低温的敏感性增强。转录谱分析表明，gdpS突变影响了188个基因的转录（92个基因上调，96个基因下调）。具体而言，负责甘油代谢的基因最显着上调和突变株中的大多数改变的基因是那些参与氮代谢。此外，最显著下调的基因包括betB基因，其产物催化甘氨酸甜菜碱的合成并赋予耐寒性。初步结果表明，gdpS基因可能参与了S.表皮细胞在面对不利的环境因素时，可能通过调节能量代谢基因的表达来实现。此外，gdpS基因对S.表皮细胞在低温下存活的可能机制可能与其对betB基因表达的影响有关。在线版本包含补充材料，可通过10.1186/s12866-022-02708-6获得。
In the genome of staphylococci, only the gdpS gene encodes the conserved GGDEF domain, which is the characteristic of diguanylate cyclases. In our previous study, we have demonstrated that the gdpS gene can modulate biofilm formation by positively regulating the expression of ica operon in Staphylococcus epidermidis. Moreover, this regulation seems to be independent of the c-di-GMP signaling pathway and the protein-coding function of this gene. Therefore, the biological function of the gdpS gene remains to be further investigated. In the present study, it was observed that mutation of the gdpS gene induced S. epidermidis to enter into a presumed viable but nonculturable state (VBNC) after cryopreservation with glycerol. Similarly, when moved from liquid to solid culture medium, the gdpS mutant strain also exhibited a VBNC state. Compared with the wild-type strain, the gdpS mutant strain autolyzed more quickly during storage at 4℃, indicating its increased susceptibility to low temperature. Transcriptional profiling analysis showed that the gdpS mutation affected the transcription of 188 genes (92 genes were upregulated and 96 genes were downregulated). Specifically, genes responsible for glycerol metabolism were most markedly upregulated and most of the altered genes in the mutant strain are those involved in nitrogen metabolism. In addition, the most significantly downregulated genes included the betB gene, whose product catalyzes the synthesis of glycine betaine and confers tolerance to cold. The preliminary results suggest that the gdpS gene may participate in VBNC formation of S. epidermidis in face of adverse environmental factors, which is probably achieved by regulating expression of energy metabolism genes. Besides, the gdpS gene is critical for S. epidermidis to survive low temperature, and the underlying mechanism may be partly explained by its influence on expression of betB gene. The online version contains supplementary material available at 10.1186/s12866-022-02708-6.
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影响因子：
3.2
作者：
Fedtke, I;Kamps, A;Götz, F
通讯作者：
Götz, F
影响因子：
2.8
作者：
Fast, B;Lindgren, PE;Gotz, F
通讯作者：
Gotz, F
影响因子：
3.6
作者：
Heilmann, C;Schweitzer, O;Gotz, F
通讯作者：
Gotz, F
DOI：
10.1038/nrmicro.2017.99
发表时间：
2017-12
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
Koo H;Allan RN;Howlin RP;Stoodley P;Hall-Stoodley L
通讯作者：
Hall-Stoodley L
影响因子：
3.2
作者：
KO, R;SMITH, LT;SMITH, GM
通讯作者：
SMITH, GM