Molecular characterization of mutations induced by gamma irradiation in rice
Molecular characterization of mutations induced by gamma irradiation in rice
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DOI：
10.1266/ggs.84.361
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发表时间：
2009-10-01
影响因子：
1.1
通讯作者：
Nishimura, Minoru
中科院分区：
文献类型：
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作者：
Morita, Ryouhei;Kusaba, Makoto;Nishimura, Minoru
为了分析伽玛辐射对高等植物的诱导突变，我们对水稻进行伽玛辐射，筛选表达糯乳（wx）、叶绿素b缺乏症、胚乳蛋白缺乏症、赤霉素相关矮化症和缩短质体时间表型的突变，以明确突变类型。核苷酸序列分析表明，伽玛射线诱导的突变最常见的是缺失，尤其是小缺失。24个突变中，15个为小缺失（1 ~ 16 bp）， 4个为大缺失（9.4 ~ 129.7 kbp）， 3个为单碱基替换，2个为反转。没有发现长度为100 bp-8 kbp的缺失，这表明伽马辐射不太可能诱导100 bp至8 kbp的缺失，而更有可能诱导1至10 bp或10 kbp左右或更多的缺失。基于这些结果，通过在靶向诱导局部损伤基因组（TILLING）中使用错配切割分析来检测小的缺失和碱基替换，或者通过阵列比较基因组杂交（aCGH）来检测大的缺失，可以将反向遗传学应用于伽马辐照诱导的水稻缺失。
In order to analyze mutations induced by gamma irradiation in higher plants, we irradiated rice with gamma rays and screened for mutations expressing phenotypes of glutinous endosperm (wx), chlorophyll b deficiency, endosperm protein deficiency, gibberellin-related dwarfism, and shortened plastochron in order to clarify types of mutations. Nucleotide sequence analysis showed that the most frequent mutation induced by gamma rays was deletion, particularly small deletion. Of the 24 mutations, 15 were small deletions (1-16 bp), four were large deletions (9.4-129.7 kbp), three were single-base substitutions, and two were inversions. Deletions 100 bp-8 kbp in length were not found, suggesting that gamma irradiation is unlikely to induce deletions of 100 bp to 8 kbp but is more likely to induce deletions between 1 and several ten bp or those of around 10 kbp or more. Based on the results, reverse genetics applications may be possible for gamma irradiation-induced deletions in rice by mismatch cleavage analysis used in Targeting Induced Local Lesions IN Genomes (TILLING) to detect small deletions and base substitutions or by using array comparative genomic hybridization (aCGH) to detect large deletions.