Manipulation of temperature and illumination conditions for enhanced β-carotene production by mutant 32 of Rhodotorula glutinis
Manipulation of temperature and illumination conditions for enhanced β-carotene production by mutant 32 of Rhodotorula glutinis
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DOI：
10.1046/j.1472-765x.2002.01095.x
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发表时间：
2002-01-01
影响因子：
2.4
通讯作者：
Gadre, RV
中科院分区：
文献类型：
--
作者：
Bhosale, P;Gadre, RV
目的：利用温度和光照条件提高粘红曲菌高产突变体32的β -胡萝卜素产量。方法与结果：在不同温度和光照条件下，在1升发酵罐中研究生长和β -胡萝卜素的产生。生长和β -胡萝卜素的最适温度分别为30℃和20℃。在30℃下，72 h β -胡萝卜素产量为125 +/- 2 mg l(-1)，占类胡萝卜素总量的66%；在20℃时，其含量为250 +/- 7 mg l(-1)，占总类胡萝卜素含量的92%。用1000倍白光连续照射发酵罐阻碍了生长和类胡萝卜素的合成。在30°c时，在对数后期照亮发酵罐导致β -胡萝卜素产量增加58%，同时甲苯产量减少；然而，在20度时，它没有明显的增加。研究的意义和影响：适当的培养条件可以提高粘粒红霉β -胡萝卜素的产量，使其成为β -胡萝卜素的重要来源。
Aims: Enhancement in the production of beta-carotene by the hyper producer mutant 32 of Rhodotorula glutinis by manipulation of temperature and illumination.Methods and Results: Growth and beta-carotene production was investigated in a 1 litre fermenter at different temperature and illumination conditions. The optimum temperature for growth and beta-carotene production was 30 and 20degreesC, respectively. At 30degreesC, beta-carotene production was 125 +/- 2 mg l(-1) and accounted for 66% of the total carotenoids in 72 h; at 20degreesC, it was 250 +/- 7 mg l(-1) and accounted for 92% of total carotenoid content. Continuous illumination of the fermenter by 1000 1x white light hampered growth as well as carotenoid synthesis. At 30degreesC, illuminating the fermenter in late logarithmic phase resulted in a 58% increase in beta-carotene production with a concurrent decrease in torulene; at 20degreesC, however, it showed no appreciable increase.Significance and Impact of the Study: Proper manipulation of culture conditions enhanced beta-carotene production by R. glutinis which makes it a significant source of beta-carotene.