Analogs of guanine nucleoside triphosphates for sequencing applications
Analogs of guanine nucleoside triphosphates for sequencing applications
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DOI：
10.1081/ncn-100002325
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发表时间：
2001-01-01
影响因子：
1.3
通讯作者：
Kumar, S
中科院分区：
文献类型：
--
作者：
McDougall, MG;Sun, L;Kumar, S
我们已经合成了超过30种不同的脱氧核糖核苷和三磷酸，其在碱基或磷酸部分中具有修饰，作为用于DNA测序应用的2 '-dGTP的类似物。所有修饰的核苷三磷酸都作为DNA聚合酶的底物进行测试，包括SequenaseTM T7 DNA聚合酶或ThermoSequenaseTM DNA聚合酶。其中两种类似物7-乙基-7-脱氮-dGTP和7-羟甲基-7-脱氮-dGTP符合我们作为更好测序试剂的要求。
We have synthesized more than 30 different deoxyribonucleosides and triphosphates with modifications either in the base or the phosphate moiety as analogs of 2'-dGTP for DNA sequencing applications. All the modified nucleoside triphosphates were tested as substrates for DNA polymerases, including Sequenase(TM) T7 DNA polymerase or Thermo Sequenase(TM) DNA polymerase. Two of the analogs, 7-ethyl-7-deaza-dGTP and 7-hydroxymethyl-7-deaza-dGTP meet our requirements as better sequencing reagents.