Tamoxifen-inducible glia-specific Cre mice for somatic mutagenesis in oligodendrocytes and Schwann cells
Tamoxifen-inducible glia-specific Cre mice for somatic mutagenesis in oligodendrocytes and Schwann cells
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DOI：
10.1016/s1044-7431(03)00029-0
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发表时间：
2003-04-01
影响因子：
3.5
通讯作者：
Suter, U
中科院分区：
文献类型：
--
作者：
Leone, DP;Genoud, S;Suter, U
诱导转基因为体内基因功能分析提供了一种有价值的技术。我们报告了携带神经胶质细胞谱系特异性转基因的小鼠品系的生成和表征，这些转基因表达他莫昔芬诱导型 Cre 重组酶的改进变体 CreERT2，其中重组酶与人雌激素受体的突变配体结合结构域融合。使用 PLP-CreERT2 转基因，我们生成了表现出特定可诱导 Cre 功能的小鼠，通过与 Rosa26 Cre-LacZ 报告系、发育中和成体 Schwarm 细胞、成熟髓鞘少突胶质细胞以及成体中未分化的 NG2 阳性少突胶质细胞前体的杂交实验进行分析。使用 P0Cx-CreERT2 转基因，我们还建立了具有诱导型 Cre 功能的小鼠品系，特别是在 Schwarm 细胞谱系中。这些他莫昔芬诱导的 CreERT2 系将允许对基于 loxP 的条件突变小鼠的发育中和成年雪旺细胞以及少突胶质细胞谱系的基因功能进行详细的时空控制分析。 (C) 2003 年爱思唯尔科学（美国）。版权所有。
Inducible transgenesis provides a valuable technique for the analysis of gene function in vivo. We report the generation and characterization of mouse lines carrying glia lineage-specific transgenes expressing an improved variant of the tamoxifen-inducible Cre recombinase, CreERT2, where the recombinase is fused to a mutated ligand binding domain of the human estrogen receptor. Using a PLP-CreERT2 transgene, we have generated mice that show specific inducible Cre function, as analyzed by cross-breeding experiments into the Rosa26 Cre-LacZ reporter line, in developing and adult Schwarm cells, in mature myelinating oligodendrocytes, and in undifferentiated NG2-positive oligodendrocyte precursors in the adult. Using a P0Cx-CreERT2 transgene, we have also established mouse lines with inducible Cre function specifically in the Schwarm cell lineage. These tamoxifen-inducible CreERT2 lines will allow detailed spatiotemporally controlled analysis of gene functions in loxP-based conditional Mutant mice in both developing and adult Schwann cells and in the oligodendrocyte lineage. (C) 2003 Elsevier Science (USA). All rights reserved.