Decreased Expression of a Novel Prostaglandin Transporter, OAT-PG, Facilitates Renocortical PGE(2) Accumulation during Rat Pregnancy
Decreased Expression of a Novel Prostaglandin Transporter, OAT-PG, Facilitates Renocortical PGE(2) Accumulation during Rat Pregnancy
复制标题
新型前列腺素转运蛋白 OAT-PG 表达下降促进大鼠妊娠期间肾皮质 PGE(2) 积累
DOI：
10.1159/000353977
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Maruyama Y
中科院分区：
文献类型：
--
作者：
Kazama I;Matsubara M;Kanai Y;Hatano R;Asano S;Endo Y;Toyama H;Ejima Y;Kurosawa S;Maruyama Y
前列腺素(PG)特异性有机阴离子转运体(OAT-PG)是近年来发现的一种参与前列腺素E2(PGE2)局部清除的肾脏转运体。由于PGE2的生物合成在妊娠期间并不增加，这种转运蛋白的表达会影响妊娠期间肾脏PGE2含量的变化。结果妊娠中晚期肾皮质PGE2浓度显著升高，最大值为(47.6±11.5)%。虽然环氧合酶和PG合成酶等酶的表达没有增加，但OAT-PG的表达在整个妊娠过程中显著下降，与肾皮质PGE2浓度的变化呈负相关。结论首次证实妊娠大鼠肾脏组织中PGE2浓度升高，这可能与妊娠大鼠肾小球滤过率升高有关。组织中PGE2含量升高可能与OAT-PG表达减少有关。
BackgroundProstaglandin (PG)-specific organic anion transporter (OAT-PG) is a recently identified renal transporter involved in the local clearance of prostaglandin E 2 (PGE 2). Since the renal biosynthesis of PGE 2 is not increased during pregnancy, this transporter expression would affect the gestational changes in the renal PGE 2 content.MethodsKidneys from rats at different gestational stages were used to examine gestational changes in the renocortical PGE 2 concentration. The renal expression of OAT-PG and the enzymes for PGE 2 synthesis was also examined sequentially, together with the gestational changes in renal renin production.ResultsThe renocortical PGE 2 concentration was significantly increased during midterm to late pregnancy, with a maximum increase of 47.6±11.5% from the virgin value. Although the expression of the enzymes, such as cyclooxygenases and PG synthases, was not increased, that of OAT-PG was significantly decreased throughout pregnancy, inversely correlating with changes in the renocortical PGE 2 concentration. Renal renin production was significantly increased during pregnancy.ConclusionThis study demonstrated for the first time that the tissue PGE 2 concentration was increased in pregnant rat kidneys, which may be associated with the gestational rise in glomerular filtration rate. The decreased expression of OAT-PG was thought to be responsible for the increased tissue PGE 2 content.