Measurement of imino 1H-1H residual dipolar couplings in RNA.
Measurement of imino 1H-1H residual dipolar couplings in RNA.
复制标题
RNA 中亚氨基 1H-1H 残留偶极耦合的测量。
DOI：
10.1007/s10858-008-9293-8
复制
发表时间：
2009
影响因子：
2.7
通讯作者：
Pardi,Arthur
中科院分区：
文献类型：
--
作者：
Latham,MichaelP;Pardi,Arthur
Imino1H-15N残留偶极耦合(RDC)提供了额外的结构信息，补充了标准的1H-1H NOE，从而改善了RNA的局部和全局结构。在这里，我们报告了铁反应元件(IRE)RNA和nativeE.ColitRNAValue的亚胺1H-1H RDC的测量。在这两个RNA中，都观察到了G-U摆动碱基对中的imino质子之间以及相邻碱基对上的imino质子之间的1H-1H RDC。这些亚胺1H-1H RDC补充了标准的1H-15N RDC，因为1H-1H矢量通常指向螺旋轴，大致垂直于1H-15N RDC。纵向弛豫增强的使用使光谱的信噪比比标准实验提高了约3.5倍。测量亚氨基1H-1H RDC的能力提供了新的限制，可用于RNA的核磁共振结构域定位和结构研究。
Imino1H–15N residual dipolar couplings (RDCs) provide additional structural information that complements standard1H–1H NOEs leading to improvements in both the local and global structure of RNAs. Here, we report measurement of imino1H–1H RDCs for the Iron Responsive Element (IRE) RNA and nativeE. colitRNAValusing a BEST-Jcomp-HMQC2 experiment.1H–1H RDCs are observed between the imino protons in G–U wobble base pairs and between imino protons on neighboring base pairs in both RNAs. These imino1H–1H RDCs complement standard1H–15N RDCs because the1H–1H vectors generally point along the helical axis, roughly perpendicular to1H–15N RDCs. The use of longitudinal relaxation enhancement increased the signal-to-noise of the spectra by ~3.5-fold over the standard experiment. The ability to measure imino1H–1H RDCs offers a new restraint, which can be used in NMR domain orientation and structural studies of RNAs.