Programmed cell death and cell necrosis activity during hyperthermic stress-induced bleaching of the symbiotic sea anemone Aiptasia sp.
Programmed cell death and cell necrosis activity during hyperthermic stress-induced bleaching of the symbiotic sea anemone Aiptasia sp.
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DOI：
10.1016/s0022-0981(02)00036-9
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发表时间：
2002-06-10
影响因子：
2
通讯作者：
Thomason, JC
中科院分区：
文献类型：
--
作者：
Dunn, SR;Bythell, JC;Thomason, JC
不同的细胞死亡途径进行了研究，在漂白过程中的海葵Aiptasia sp。在高温处理。使用一套技术，（苏木精和伊红染色的石蜡包埋的组织切片，原位末端标记（ISEL）的片段化DNA，琼脂糖凝胶电泳电子显微镜）的坏死和程序性细胞死亡（PCD）的活动都表明。在4天的处理期后，宿主内胚层组织经历坏死细胞死亡。这表现为广泛的细胞降解、细胞质和细胞器扩张、细胞肿胀和破裂、核染色质不规则固缩和大量细胞碎片。宿主细胞坏死与虫黄嘌呤释放相关，并具有正常、健康的外观进入腔肠。较长时间的高温处理（7天）与进一步的动物细胞降解和降解内胚层内残留的虫黄嘌呤原位降解相关。在同一降解的内胚层组织中，虫黄素的降解是同时发生的两种形式的细胞死亡的结果，这两种形式被鉴定为程序性细胞死亡和细胞坏死。虫黄藻细胞程序性死亡的特点是细胞质和细胞器的浓缩，细胞皱缩，在细胞壁周围形成积累体，和DNA片段化。虫黄藻细胞坏死的特点是细胞质和细胞器的扩张，细胞肿胀和溶解，分散的细胞成分碎片，和DNA片段。虫黄藻内程序性细胞死亡途径的存在对于理解珊瑚漂白事件是重要的，这一过程的演变和所涉及的细胞触发机制的激活引起了有趣的问题。(C)2002 Elsevier Science B.V.保留所有权利。
Different cell death pathways were investigated during bleaching in the sea anemone Aiptasia sp. in response to hyperthermic treatment. Using a suite of techniques, (haematoxylin and eosin staining of paraffin wax-embedded tissue sections, in-situ end labelling (ISEL) of fragmented DNA, agarose gel electrophoresis electron microscopy) both necrotic and programmed cell death (PCD) activity were indicated. After a treatment period of 4 days, the host endoderm tissues underwent necrotic cell death. This was indicated by widespread cellular degradation, dilation of cell cytoplasm and organelles, cell swelling and rupture, irregular pyknotic condensation of nuclear chromatin, and abundant cell debris. Host cell necrosis was associated with the release of zooxanthellae with a normal, healthy appearance into the coelenteron. Longer periods of hyperthermic treatment (7 days) were correlated with further animal cell degradation and the in-situ degradation of zooxanthellae remaining within the degraded endoderm. Within the same degraded endoderm tissue, the degradation of zooxanthellae resulted from two forms of cell death occurring simultaneously, which were identified as programmed cell death and cell necrosis. Programmed cell death of zooxanthellae was characterised by condensation of the cytoplasm and organelles, cell shrinkage, formation of accumulation bodies at the periphery of the cell wall, and DNA fragmentation. Cell necrosis of zooxanthellae was characterised by dilation of the cytoplasm and organelles, cell swelling and lysis, dispersion of cell component debris, and DNA fragmentation. The existence of a programmed cell death pathway within zooxanthellae is important to the understanding of coral bleaching events, raising interesting questions regarding the evolution of this process and the activation of the cellular trigger mechanisms involved. (C) 2002 Elsevier Science B.V. All rights reserved.