In silico cloning and characterization of the TGA (TGACG MOTIF-BINDING FACTOR) transcription factors subfamily in Carica papaya
In silico cloning and characterization of the TGA (TGACG MOTIF-BINDING FACTOR) transcription factors subfamily in Carica papaya
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DOI：
10.1016/j.plaphy.2012.02.011
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发表时间：
2012-05-01
影响因子：
6.5
通讯作者：
Santamaria, Jorge M.
中科院分区：
文献类型：
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作者：
Idrovo Espin, Fabio Marcelo;Peraza-Echeverria, Santy;Santamaria, Jorge M.
TGA 转录因子属于 bZIP D 组亚家族，在疾病抵抗和发育中发挥重要作用。在拟南芥中发现的大多数 TGA 与 SAR 的主调节因子 NPR1 相互作用，后者控制 PR 基因的表达。 作为确定这些转录因子可能参与木瓜防御的第一种方法，我们从 Carica papaya cv.日出。鉴定出 CpTGA1 至 CpTGA6 的六种直系同源物。预测的 CpTGA 蛋白与 AtTGA 序列高度相似，并且可能具有相同的 DNA 结合特性和转录调控特征。蛋白质序列比对证明了保守结构域的存在，这是这组转录因子的特征。系统发育表明，CpTGA 进化成与防御和花发育相关的三个不同的分支。这是通过 RT-PCR 评估番木瓜不同组织中 CpTGA 成员整个亚家族的基础表达模式的第一份报告。马拉多尔成熟植株。总体而言，CpTGA1、CpTGA3、CpTGA6 和 CpTGA4 在所有测试组织中均显示基础表达； CpTGA2 在除叶柄外的所有组织中均强烈表达，而 CpTGA5 仅在花瓣中表达，在叶柄中表达程度较低。尽管需要对花药和其他花结构进行更详细的研究，但我们认为 CpTGA5 可能具有组织特异性，并且可能参与木瓜花的发育。另一方面，我们在这里首次报告了 CpTGA 全家族对水杨酸 (SA) 的反应的表达。 CpTGA3、CpTGA4 和 CpTGA6 的表达随着 SA 的增加而增加，这表明它参与了木瓜的 SAR 反应。 (C)2012 Elsevier Masson SAS。版权所有。
The TGA transcription factors belong to the subfamily of bZIP group D that play a major role in disease resistance and development. Most of the TGA identified in Arabidopsis interact with the master regulator of SAR, NPR1 that controls the expression of PR genes.As a first approach to determine the possible involvement of these transcription factors in papaya defense, we characterized Arabidopsis TGA orthologs from the genome of Carica papaya cv. SunUp. Six orthologs CpTGA1 to CpTGA6, were identified. The predicted CpTGA proteins were highly similar to AtTGA sequences and probably share the same DNA binding properties and transcriptional regulation features. The protein sequences alignment evidenced the presence of conserved domains, characteristic of this group of transcription factors. The phylogeny showed that CpTGA evolved into three different subclades associated with defense and floral development. This is the first report of basal expression patterns assessed by RT-PCR, from the whole subfamily of CpTGA members in different tissues from papaya cv. Maradol mature plants. Overall, CpTGA1, CpTGA3 CpTGA6 and CpTGA4 showed a basal expression in all tissues tested; CpTGA2 expressed strongly in all tissues except in petioles while CpTGA5 expressed only in petals and to a lower extent in petioles. Although more detailed studies in anthers and other floral structures are required, we suggest that CpTGA5 might be tissue-specific, and it might be involved in papaya floral development. On the other hand, we report here for the first time, the expression of the whole family of CpTGA in response to salicylic acid (SA). The expression of CpTGA3, CpTGA4 and CpTGA6 increased in response to SA, what would suggest its involvement in the SAR response in papaya. (C)2012 Elsevier Masson SAS. All rights reserved.