Effect of mutated transporters associated with antigen-processing 2 on characteristic major histocompatibility complex binding peptides: analysis using electrospray ionization tandem mass spectrometry
Effect of mutated transporters associated with antigen-processing 2 on characteristic major histocompatibility complex binding peptides: analysis using electrospray ionization tandem mass spectrometry
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DOI：
10.1002/rcm.1438
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发表时间：
2004-01-01
影响因子：
2
通讯作者：
Kumagai, S
中科院分区：
文献类型：
--
作者：
Kageyama, G;Kawano, S;Kumagai, S
A novel allele of transporters associated with the antigen-processing (TAP) 2 gene, TAP2*Bky2 (VaI(577)), is significantly increased in Japanese patients with Sjogren's syndrome (SS), and has a strong association with SS-A/Ro autoantibody production in SS and autoantibody including anti-SS-A/Ro and anti-U1 RNP antibody in systemic lupus erythematosus (SLE). To determine the influence of this natural mutated TAP on peptides loaded onto MHC class I, we analyzed the repertoire of peptides loaded onto MHC class I on transfectants with TAP1 and TAP2 or mutated TAP2 by electrospray ionization tandem mass spectrometry (ESI-MS/MS). After comparison of the peptide profiles we identified three peptides from only mutated TAP transfectants. Moreover, one of these peptides is derived from snRNP A, which is a target for anti-U1 RNP antibody. To our knowledge this is the first report to show that the natural mutation of TAP2 changes the peptide profile loaded onto MHC class I molecules. Copyright (C) 2004 John Wiley Sons, Ltd.
