ASSESSMENT OF METHODS FOR MEASUREMENT OF GLYCOGEN SYNTHETASE-ACTIVITY INCLUDING A NEW DIRECT 1 STEP ASSAY
ASSESSMENT OF METHODS FOR MEASUREMENT OF GLYCOGEN SYNTHETASE-ACTIVITY INCLUDING A NEW DIRECT 1 STEP ASSAY
复制标题
DOI：
10.1016/0003-2697(73)90510-1
复制
发表时间：
1973-01-01
影响因子：
2.9
通讯作者：
ROTTENBERG, DA
中科院分区：
文献类型：
--
作者：
PASSONNEAU, JV;ROTTENBERG, DA
在粗组织制备方面，雄性Osborne-Mendel断奶大鼠在液氮中快速冷冻，组织在-2O“下解剖并称重，样品在-50”保存直到使用。在50 mM Tris-WC1缓冲液，pH 7.5，含有5 mM EDTA，25 mM KF和5 mM二硫苏糖醇的条件下，在0“的适当体积下制备匀浆。在两步化验中，将等量的组织匀浆加入10528
For crude tissue preparations, male Osborne-Mendel weanling rats were quick-frozen in liquid nitrogen, the tissues dissected and weighed at-2O”, and the samples stored at-50” until use. Homogenates were made at 0” in appropriate volumes at 50 mM Tris-WC1 buffer, pH 7.5, containing 5 mM EDTA, 25 mM KF, and 5 mM dithiothreitol. In the two-step assay, aliquots of tissue homogenates were added to 10 528