Cytotoxicity of Silver Nanoparticle and Chitin-Nanofiber Sheet Composites Caused by Oxidative Stress.
Cytotoxicity of Silver Nanoparticle and Chitin-Nanofiber Sheet Composites Caused by Oxidative Stress.
复制标题
氧化应激引起的银纳米颗粒和甲壳素纳米纤维片复合材料的细胞毒性。
DOI：
10.3390/nano6100189
复制
发表时间：
2016-10-20
期刊：
影响因子：
--
通讯作者：
Yokoe H
中科院分区：
文献类型：
--
作者：
Kinoda J;Ishihara M;Hattori H;Nakamura S;Fukuda K;Yokoe H
尺寸可控的球形银纳米颗粒（<10 nm）和几丁质-纳米片复合物（Ag NPs/CNFS）先前已被报道在体外具有强的抗微生物活性。虽然Ag NPs/CNFS具有很强的抗菌活性，但其细胞毒性尚未研究。进行本研究以评估Ag NPs/CNFS对体外成纤维细胞的细胞毒性和体内伤口修复的愈合延迟的影响，重点是氧化应激。利用成纤维细胞增殖试验、体外细胞裂解物的一氧化氮/二氧化氮（NO/NO2）测量、体内Ag NPs/CNFS对使用糖尿病小鼠的愈合受损的伤口修复的抑制作用、皮肤切片的8-羟基-2 ′-脱氧鸟苷（8-OHdG）免疫组织化学染色，并进行伤口中羰基蛋白的产生以评价氧化应激的细胞毒性。Ag NPs/CNFS表现出对成纤维细胞的细胞毒性，体外细胞裂解物中总NO/NO2水平显著增加，体内8-OHdG和羰基蛋白水平增加。尽管连续Ag NPs/CNFS治疗组的伤口修复延迟，但可以通过用盐水洗涤覆盖的伤口来减轻。因此，Ag NPs/CNFS可被接受为抗感染伤口敷料。
Size-controlled spherical silver nanoparticles (<10 nm) and chitin-nanofiber sheet composites (Ag NPs/CNFS) have previously been reported to have strong antimicrobial activity in vitro. Although Ag NPs/CNFS have strong antimicrobial activity, their cytotoxicity has not been investigated. This study was performed to evaluate the effects of Ag NPs/CNFS on cytotoxicity for fibroblasts in vitro and healing delay of wound repair in vivo, focused on oxidative stress. Cytotoxic activities of Ag NPs/CNFS were investigated using a fibroblast cell proliferation assay, nitric oxide/nitrogen dioxide (NO/NO2) measurement of the cell lysates in vitro, inhibitory effects of Ag NPs/CNFS on healing-impaired wound repair using diabetic mice in vivo, 8-hydroxy-2′-deoxyguanosine (8-OHdG) immunohistochemical staining of the skin sections, and generation of carbonyl protein in the wound was performed to evaluate cytotoxicity with oxidative stress. Ag NPs/CNFS exhibited cytotoxicity for fibroblasts and a significant increase of total NO/NO2 levels in the cell lysates in vitro and increased levels of 8-OHdG and carbonyl proteins in vivo. Although wound repair in the continuously Ag NPs/CNFS-treated group was delayed, it could be mitigated by washing the covered wound with saline. Thus, Ag NPs/CNFS may become accepted as an anti-infectious wound dressing.