Spectrophotometric photodynamic detection involving extracorporeal treatment with hexaminolevulinate for bladder cancer cells in voided urine.
Spectrophotometric photodynamic detection involving extracorporeal treatment with hexaminolevulinate for bladder cancer cells in voided urine.
复制标题
分光光度光动力检测涉及用六氨基乙酰丙酸盐对排泄尿液中的膀胱癌细胞进行体外处理。
DOI：
10.1007/s00432-017-2476-5
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Fujimoto K.
中科院分区：
文献类型：
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作者：
Nakai Y;Ozawa T;Mizuno F;Onishi S;Owari T;Hori S;Morizawa Y;Tatsumi Y;Miyake M;Tanaka N;Tsuruta D;Fujimoto K.
PurposeTo评估的可行性hexaminolevulinate（HAL）的光动力学检测癌细胞在排尿urin.MethodsThis研究包括50例膀胱癌，经尿道电切术（膀胱癌组）和50例门诊患者的病史没有尿路上皮癌或癌症相关的发现（无恶性组）后，组织学证实。将三分之一的排泄尿液样品与氨基乙酰丙酸孵育（ALA处理的样品），三分之一与HAL孵育（HAL处理的样品），其余样品在未处理的情况下孵育（未处理的样品）。为了检测细胞原卟啉IX水平，使用分光光度计测量样品在405 nm激发波长下的强度。结果HAL诱导的荧光细胞学（HFC）显示，高级别肿瘤患者的差异显著高于低级别肿瘤患者（p = 0.0003），低级别肿瘤患者的差异显著高于无尿路上皮癌或癌症相关发现史的患者（p = 0.021）。ALA诱导荧光细胞学（AFC）和HFC的受试者工作特征曲线下的面积分别为0.77和0.81。HFC的AUC显著高于AFC（p <0.0001）。常规细胞学、AFC和HFC的总体敏感性值分别为49%、74%和74%。AFC和HFC的总体特异性值分别为70和94%，respectively. ConclusionsSpectrophotometric photodynamic detection involving extracorporeal treatment with HAL for bladder cancer cells in voided urine显示出很高的准确性。这种膀胱癌检测方法简单，成本效益高，具有临床应用的潜力。
PurposeTo evaluate the feasibility of hexaminolevulinate (HAL) for the photodynamic detection of cancer cells in voided urine.MethodsThis study included 50 patients with bladder cancer that was confirmed histologically after transurethral resection (bladder cancer group) and 50 outpatients without a history of urothelial carcinoma or cancer-related findings (no malignancy group). One third of the voided urine samples were incubated with aminolevulinic acid (ALA-treated samples), one third were incubated with HAL (HAL-treated samples), and the remaining samples were incubated without treatment (untreated samples). For detecting cellular protoporphyrin IX levels, the intensity of the samples at the excitation wavelength of 405 nm was measured using a spectrophotometer. The difference between the intensity of the ALA-treated or HAL-treated samples and the untreated samples at 635 nm was calculated.ResultsHAL-induced fluorescence cytology (HFC) showed that the difference was significantly higher in patients with high-grade tumors than in those with low-grade tumors (p= 0.0003) and the difference was significantly higher in patients with low-grade tumors than in those without a history of urothelial carcinoma or cancer-related findings (p= 0.021). The areas under the receiver operating characteristic curves of ALA-induced fluorescence cytology (AFC) and HFC were 0.77 and 0.81, respectively. The AUC of HFC was significantly higher than that of AFC (p< 0.0001). The overall sensitivity values for conventional cytology, AFC, and HFC were 49, 74, and 74%, respectively. The overall specificity values for AFC and HFC were 70 and 94%, respectively.ConclusionsSpectrophotometric photodynamic detection involving extracorporeal treatment with HAL for bladder cancer cells in voided urine showed high accuracy. This bladder cancer detection method is easy and cost-effective, and has the potential for clinical use.