Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex
Somatostatin receptors (SSTR1-5) on inhibitory interneurons in the barrel cortex
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DOI：
10.1007/s00429-019-02011-7
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发表时间：
2019-12-23
影响因子：
3.1
通讯作者：
Kossut, Malgorzata
中科院分区：
文献类型：
--
作者：
Lukomska, Agnieszka;Dobrzanski, Grzegorz;Kossut, Malgorzata
大脑皮层中的抑制性中间神经元含有特定中间神经元亚型特有的特定蛋白质或肽。在小鼠中，三种生化标记物构成非重叠的中间神经元群体，占所有抑制细胞的 80-90%。这些中间神经元表达小清蛋白 (PV)、生长抑素 (SST) 或血管活性肠肽 (VIP)。 SST不仅是特定中间神经元亚型的标志物，也是一种重要的神经肽，通过生长抑素受体（SSTR1-5）参与大脑中的众多生化和信号传导通路。在神经系统中，SST 充当神经调节剂和神经递质，影响记忆、学习和情绪等。在感觉皮层中，GABA 和 SST 共定位于大约 30% 的中间神经元中。考虑到抑制性中间神经元之间相互作用在皮质可塑性中的重要性以及可能的 GABA 和 SST 共同释放，研究不同 SSTR 在皮质中间神经元上的定位似乎很重要。在这里，我们检查了 SSTR1-5 在含有 PV、SST 或 VIP 的桶状皮层中间神经元上的分布。使用特定抗体对转基因小鼠的脑切片进行免疫荧光染色，这些转基因小鼠在一种特定的中间神经元亚型（PV-Ai14、SST-Ai14 和 VIP-Ai14 小鼠）中表达红色荧光。 PV、SST 和 VIP 中间神经元上的 SSTR 表达在皮质层之间存在差异，我们发现 SSTR 在桶状皮层 L4 中分布有两种模式。我们还证明，与其他中间神经元相比，PV 细胞不表达 SSTR2，但表达其他 SSTR。 SST 中间神经元之间没有发现化学突触，但它们表达所有五种 SSTR 亚型。
Inhibitory interneurons in the cerebral cortex contain specific proteins or peptides characteristic for a certain interneuron subtype. In mice, three biochemical markers constitute non-overlapping interneuron populations, which account for 80-90% of all inhibitory cells. These interneurons express parvalbumin (PV), somatostatin (SST), or vasoactive intestinal peptide (VIP). SST is not only a marker of a specific interneuron subtype, but also an important neuropeptide that participates in numerous biochemical and signalling pathways in the brain via somatostatin receptors (SSTR1-5). In the nervous system, SST acts as a neuromodulator and neurotransmitter affecting, among others, memory, learning, and mood. In the sensory cortex, the co-localisation of GABA and SST is found in approximately 30% of interneurons. Considering the importance of interactions between inhibitory interneurons in cortical plasticity and the possible GABA and SST co-release, it seems important to investigate the localisation of different SSTRs on cortical interneurons. Here, we examined the distribution of SSTR1-5 on barrel cortex interneurons containing PV, SST, or VIP. Immunofluorescent staining using specific antibodies was performed on brain sections from transgenic mice that expressed red fluorescence in one specific interneuron subtype (PV-Ai14, SST-Ai14, and VIP-Ai14 mice). SSTRs expression on PV, SST, and VIP interneurons varied among the cortical layers and we found two patterns of SSTRs distribution in L4 of barrel cortex. We also demonstrated that, in contrast to other interneurons, PV cells did not express SSTR2, but expressed other SSTRs. SST interneurons, which were not found to make chemical synapses among themselves, expressed all five SSTR subtypes.