miR-1247-3p mediates apoptosis of cerebral neurons by targeting caspase-2 in stroke
miR-1247-3p mediates apoptosis of cerebral neurons by targeting caspase-2 in stroke
复制标题
miR-1247-3p通过靶向中风中的caspase-2介导脑神经元凋亡
DOI：
10.1016/j.brainres.2019.02.020
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发表时间：
2019-07-01
期刊：
影响因子：
2.9
通讯作者：
Yuan, Qiong
中科院分区：
文献类型：
--
作者：
Zhang, Rong;Zhou, Weipin;Yuan, Qiong
Brain stroke is one of the leading causes of death worldwide. We explored a potential stroke-related role for a newly found microRNA, miR-1247-3p, and one of its target genes, caspase-2, predicted by TargetScanVert. In the present study, we found that miR-1247-3p was downregulated during ischemia/reperfusion (I/R) and that LV-miR-1247-3p overexpression attenuated brain impairment induced by I/R. Similar results were observed in neuro2a (N2a) cells treated with oxygen -glucose deprivation/reoxygenation (OGD/R). Caspase-2 was upregulated in the I/R and OGD/R model, while Z-VDVAD-FMK the inhibitor of caspase-2-inhibited apoptosis of N2a cells induced by OGD/R. An miR-1247-3p mimic inhibited caspase-2 expression and attenuated apoptosis of N2a cells induced by OGD/R. Myocardin-related transcription factor-A (MRTF-A) overexpression upregulated miR-1247 and mature miR-1247-3p levels and attenuated apoptosis induced by OGD/R, whereas its anti-apoptotic function could be blocked by a miR-1247-3p inhibitor. Hence, we conclude that miR-1247-3p may protect cells during brain stroke. This study offers insights for the development of effective therapeutics for promoting the survival of cerebral neurons during brain I/R injury.
