The sodium-glucose cotransporter 2 inhibitor luseogliflozin can suppress muscle atrophy in Db/Db mice by suppressing the expression of foxo1
The sodium-glucose cotransporter 2 inhibitor luseogliflozin can suppress muscle atrophy in Db/Db mice by suppressing the expression of foxo1
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DOI：
10.3164/jcbn.18-114
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发表时间：
2019-07-01
影响因子：
2.4
通讯作者：
Fukui, Michiaki
中科院分区：
文献类型：
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作者：
Okamura, Takuro;Hashimoto, Yoshitaka;Fukui, Michiaki
我们研究了葡萄糖共转运蛋白-2抑制剂（SGLT-2i）糖格列净对骨骼肌的影响。8周龄小鼠分别饲喂标准饮食或添加糖格列净的标准饮食8周。将小鼠分为未添加SGLT-2i的Db/m组、添加SGLT-2i抑制剂的Db/m组、未添加SGLT-2i的Db/Db组和添加SGLT-2i的Db/Db组。在注射SGLT-2i和不注射SGLT-2i的小鼠中，Db/Db小鼠比目鱼和足底肌与体重的比例显著低于Db/m小鼠。未添加SGLT-2i的Db/Db小鼠比目鱼肌横截面积显著高于添加SGLT-2i的Db/Db小鼠。Db/Db组小鼠比目鱼肌中foxol的表达量显著高于Db/m组小鼠，SGLT-2i组小鼠foxol的表达量显著低于未添加SGLT-2i组小鼠。注射SGLT-2i的Db/Db小鼠foxol的荧光强度明显低于未注射SGLT-2i的Db/Db小鼠。糖格列净抑制了Db/Db小鼠比目鱼肌中foxol表达的增加和肌肉横截面积的减少。
We investigated the effect of the sodium glucose cotransporter-2 inhibitor (SGLT-2i) luseogliflozin on skeletal muscle. Eight-weekold mice were fed a standard diet or the standard diet with added luseogliflozin for 8 weeks. The mice were divided into the following four genotype/dietary groups: Db/m mice without SGLT-2i, Db/m mice with SGLT-2i inhibitor, Db/Db without SGLT-2i, and Db/Db with SGLT-2i. Among the mice with and without SGLT-2i, the ratio of soleus and plantaris muscle to body weight in the Db/Db mice was significantly lower than that in the Db/m mice. The cross-sectional area of soleus muscle in the Db/Db mice without SGLT-2i was significantly higher than that in the Db/Db mice with SGLT-2i. The expression of foxol in soleus muscle of the Db/Db mice was significantly higher than that of the Db/m mice, and the foxol expression of the Db/Db mice with SGLT-2i was significantly lower than that of the mice without SGLT-2i. The fluorescence intensity of foxol in the Db/Db mice fed SGLT-2i was significantly lower than that in the Db/Db mice without SGLT-2i. The administration of luseogliflozin resulted in the suppression of both the increased foxol expression and the reduced muscle cross-sectional area in the soleus muscle of Db/Db mice.