The neuropeptide FMRFamide can protect cells against apoptosis in the snail digestive gland
The neuropeptide FMRFamide can protect cells against apoptosis in the snail digestive gland
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DOI：
10.1007/s10495-006-3391-4
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发表时间：
2006-02
期刊：
影响因子：
7.2
通讯作者：
Tamás Röszer;J. Kappelmayer;G. Nagy;Szentmiklósi Aj;A. Basnakian;G. Banfalvi
中科院分区：
文献类型：
--
作者：
Tamás Röszer;J. Kappelmayer;G. Nagy;Szentmiklósi Aj;A. Basnakian;G. Banfalvi
FMRFamide相关肽是调节躯体或内脏运动活动的广泛的神经递质或神经激素。最近的研究表明，这些神经肽可能参与细胞增殖和凋亡的调控。在这项工作中，我们研究了FMRFamide对无脊椎动物型增殖组织中细胞活力的可能影响。作为一个模型，我们使用的中肠腺的蜗牛，HellucorumLinnaeus。免疫组化显示含FMRFamide的神经纤维直接支配腺细胞。用50 μM FMRFamide注射蜗牛的中肠腺，用无菌去离子水或牛血清白蛋白（BSA）注射对照。注射4次。透射电子显微镜，膜联蛋白V-标记，噻唑蓝（MTT）活力试验和倍性分析进行定义的活/死细胞的组织样品中的比例。FMRFamide增加组织的MTT降低，减少凋亡核和膜联蛋白V标记的细胞的量。去离子水或BSA注射诱导细胞死亡。细胞周期分析显示，FMRFamide显著增加G 0/G1期细胞数量，但不诱导有丝分裂。我们的结论是，FMRFamide可以是细胞的生命信号，保护它们免于凋亡而不改变有丝分裂。
FMRFamide-related peptides are widespread neurotransmitters or neurohormones regulating somatic or visceral motor activity. Some recent data indicate that these neuropeptides may be involved in the control of cell proliferation and apoptosis. In this work we investigated the possible effect of FMRFamide on cell viability in an invertebrate-type proliferating tissue. As a model, we used the midintestinal gland of the snail,Helix lucorumLinnaeus. Immunohistochemistry demonstrated the direct innervation of the gland cells by FMRFamide-containing nerve fibers. Midintestinal glands of snails were injected with 50 μM FMRFamide and the control with sterile deionised water or bovine serum albumin (BSA). Injections were administrated 4 times. Transmission electron microscopy, annexin V-labeling, thiazolyl blue (MTT) viability tests and ploidy analyses were carried out to define the viable/dead cell ratio in the tissue samples. FMRFamide increased the MTT-reduction of tissues, reduced the amount of apoptotic nuclei and annexin V-labeled cells. Deionised water or BSA injection induced cell death. Cell cyle analysis revealed that FMRFamide significantly elevated the amount of cells in G0/G1phase, but did not induce mitosis. We conclude, that the FMRFamide can be a life-signal for cells, protect them from apoptosis without altering mitosis.