FLUORESCENT MODIFICATION OF ADENOSINE-CONTAINING COENZYMES - BIOLOGICAL-ACTIVITIES AND SPECTROSCOPIC PROPERTIES
FLUORESCENT MODIFICATION OF ADENOSINE-CONTAINING COENZYMES - BIOLOGICAL-ACTIVITIES AND SPECTROSCOPIC PROPERTIES
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DOI：
10.1021/bi00769a001
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发表时间：
1972-01-01
期刊：
影响因子：
2.9
通讯作者：
BARRIO, JR
中科院分区：
文献类型：
--
作者：
SECRIST, JA;WEBER, G;BARRIO, JR
John A. Secrist、Jorge R. Barrio、§ Nelson J. Leonard,* 和 Gregorio Weber 摘要：此处描述了腺苷和胞苷的荧光衍生物的合成，通过与氯乙醛在水溶液中在温和的 pH 和温度下反应，分别产生 1, 7V6-乙烯基腺苷盐酸盐和 3, 7V4-乙烯基胞苷盐酸盐。还合成了 3'-AMP、5'-AMP、3'、5'-环 AMP、ADP、ATP 和 NAD+ 的类似衍生物。对高荧光腺苷衍生物的光谱特性的观察包括 1, 7V6-乙烯-腺苷的发射最大值约为 1, 7V6-乙烯-腺苷。在 pH 7.0 的缓冲水溶液中，波长为 415 nm（校正），量子产率为 0.56，荧光寿命为 20 纳秒。荧光变化
John A. Secrist, Jorge R. Barrio, § Nelson J. Leonard,* and Gregorio Weber abstract: The synthesis of fluorescent derivatives of adeno-sine and cytidine, by reaction with chloroacetaldehyde in aqueous solution at mild pH and temperature, yielding 1, 7V6-ethenoadenosine hydrochloride and 3, 7V4-ethenocytidine hydrochloride, respectively, is here described. Analogous derivatives of 3'-AMP, 5'-AMP, 3', 5'-cyclic AMP, ADP, ATP, and NAD+ were also synthesized. Observation of the spectroscopic properties of the highly fluorescentadenosine derivatives included the emission maximum for 1, 7V6-etheno-adenosine at ca. 415 nm (corrected) in bufferedaqueous solu-tion at pH 7.0, a quantum yield of 0.56, and a fluorescence lifetime of 20 nsec. Variations in fluorescence with respect