A new ligand binding to G-G mismatch having improved thermal and alkaline stability.
A new ligand binding to G-G mismatch having improved thermal and alkaline stability.
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DOI：
10.1016/j.bmcl.2004.11.003
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发表时间：
2005-01
影响因子：
2.7
通讯作者：
T. Peng;Takashi Murase;Yuki Goto;A. Kobori;K. Nakatani
中科院分区：
文献类型：
--
作者：
T. Peng;Takashi Murase;Yuki Goto;A. Kobori;K. Nakatani
萘啶二聚体 (ND) 特异性结合双链 DNA 中的鸟嘌呤-鸟嘌呤 (G-G) 错配。为了提高配体的热稳定性和碱稳定性以及结合能力，我们研究了连接体的结构修饰。具有 2-氨基-1,8-萘啶和氨基甲酸酯连接基的新配体 (NNC) 比 ND 具有更高的热稳定性。 80℃时NNC的半衰期比ND长2.5倍。在碱性条件下，NNC 也比 ND 稳定得多。此外，NNC 与 G-G 错配的结合比 ND 更强。改进的稳定性以及 NNC 与 G-G 不匹配的结合将适合 NNC 固定传感器的实际使用。
Naphthyridine dimer (ND) specially binds to guanine–guanine (G–G) mismatch in duplex DNA. In order to improve the thermal and alkaline stability and binding ability of the ligand, we have examined structural modification of the linker. A new ligand (NNC) possessing 2-amino-1,8-naphthyridines and a carbamate linker is much more thermally stable than ND. The half-life of NNC is 2.5 times longer than that of ND at 80°C. NNC is also much more stable than ND under alkaline conditions. In addition, NNC binds to G–G mismatch more strongly than ND. The improved stability and the binding of NNC to the G–G mismatch would be suitable for the practical use of NNC-immobilized sensor.