Proteomic Analysis of Proteins Associated with Inhibition ofPseudomonas aeruginosaResistance to Imipenem Mediated by the Chinese Herbal Medicine Qi Gui Yin
Proteomic Analysis of Proteins Associated with Inhibition ofPseudomonas aeruginosaResistance to Imipenem Mediated by the Chinese Herbal Medicine Qi Gui Yin
复制标题
芪归饮抑制铜绿假单胞菌亚胺培南耐药相关蛋白质组学研究
DOI：
10.1089/mdr.2020.0110
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发表时间：
2020-09-14
影响因子：
2.6
通讯作者：
Liu, Qingquan
中科院分区：
文献类型：
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作者：
Ding, Junying;Gao, Xiang;Liu, Qingquan
目的：铜绿假单胞菌（PA）的耐药性降低了目前肺炎治疗的有效性，是一个日益严重的问题。芪归饮是一种中草药，已被用于提高抗生素治疗对耐药性细菌的疗效。本研究旨在阐明芪归饮抑制铜绿假单胞菌耐药性的作用机制。方法：采用色谱法对芪归饮的有效成分进行分析。使用相对和绝对定量的等压标签（iTRAQ）技术来比较用芪归饮治疗和未用芪归饮治疗的大鼠血清中培养的PA菌株的蛋白质表达谱。进行定量聚合酶链反应（qPCR）分析以检测基因表达变化。结果：蛋白质组学分析鉴定出76个经芪归饮治疗和未治疗的大鼠血清中培养的PA株差异表达蛋白。生物信息学分析显示，最大数量的差异表达蛋白与抗性机制相关，如群体感应，细菌生物膜形成和主动泵送。此外，qPCR分析证实，下调UandarcA基因表达与芪归饮治疗有关。结论：芪归饮大鼠血清能有效抑制铜绿假单胞菌的耐药性。绿原酸和黄芪甲苷是芪归饮的主要成分，可能通过抑制抗生素耐药性而发挥作用。我们的研究结果为涉及中草药的策略提供了新的见解，这些策略可用于治疗由耐药性细菌引起的肺炎。
Objective:Antibiotic resistance ofPseudomonas aeruginosa(PA) that lowers the effectiveness of current treatments for pneumonia is a growing problem. Qi Gui Yin is a Chinese herbal medicine that has been used to improve the efficacy of antibiotic therapy against antibiotic-resistant bacteria. This study aimed to elucidate the mechanism by which Qi Gui Yin inhibits antibiotic resistance of PA. Methods:Active components of Qi Gui Yin were analyzed by chromatography. Isobaric Tags for Relative and Absolute Quantification (iTRAQ) technology was used to compare protein expression profiles of PA strains cultured in serum from rats that were and were not treated with Qi Gui Yin. Quantitative polymerase chain reaction (qPCR) analysis was performed to detect gene expression changes. Results:Proteomic analysis identified 76 differentially expressed proteins between PA strains cultured in serum from rats that were or were not treated with Qi Gui Yin. Bioinformatics analysis revealed that the largest number of differentially expressed proteins were associated with resistance mechanisms such as quorum sensing, bacterial biofilm formation, and active pumping. In addition, qPCR analysis confirmed that downregulation ofiscUandarcAgene expression was associated with Qi Gui Yin treatment. Conclusions:Serum from Qi Gui Yin-treated rats could effectively inhibit antibiotic resistance of PA. Chlorogenic acid and astragaloside IV are the main components of Qi Gui Yin, which may mediate inhibition of antibiotic resistance. Our findings provide new insights into strategies involving Chinese herbal medicine that can be used to treat pneumonia caused by antibiotic-resistant bacteria.