Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis
Tyrosine-sulfated glycopeptide involved in cellular proliferation and expansion in Arabidopsis
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DOI：
10.1073/pnas.0706403104
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发表时间：
2007-11-13
影响因子：
11.1
通讯作者：
Matsubayashi, Yoshikatsu
中科院分区：
文献类型：
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作者：
Amano, Yukari;Tsubouchi, Hiroko;Matsubayashi, Yoshikatsu
翻译后修饰可以赋予肽特殊的功能。基于针对酪氨酸硫酸化肽的详尽液相色谱质谱分析，我们在拟南芥细胞悬浮培养基中鉴定出18-aa酪氨酸硫酸化糖肽。我们将这种肽命名为 PSY1，在纳摩尔浓度下可显着促进细胞增殖和扩张。 PSY1在各种拟南芥组织中广泛表达，包括茎尖分生组织，并且在受伤时高度上调。 PSY1 的感知取决于 At1g72300，At1g72300 是一种富含亮氨酸的重复受体激酶 (LRR-RK)，其两个旁系同源物参与植物硫因子 (PSK) 的感知，植物硫因子是一种主要促进细胞增殖的 5-aa 酪氨酸硫酸化肽。与单个破坏体相比，这三个旁系同源 LRR-RK 中的多个功能丧失突变显着增强了表型，表明这些 LRR-RK 具有重叠的功能。这些LRR-RKs的三重突变导致侏儒症，因为细胞数量和细胞大小减少，并导致受伤后组织修复不足。目前的结果表明，这个旁系同源 LRR-RK 家族整合了由两种结构不同的硫酸化肽：PSY1 和 PSK 介导的生长促进信号。
Posttranslational modification can confer special functions to peptides. Based on exhaustive liquid chromatography mass spectrometry analysis targeting tyrosine-sulfated peptides, we identified an 18-aa tyrosine-sulfated glycopeptide in Arabidopsis cell suspension culture medium. This peptide, which we named PSY1, significantly promotes cellular proliferation and expansion at nanomolar concentrations. PSY1 is widely expressed in various Arabidopsis tissues, including shoot apical meristem, and is highly up-regulated by wounding. Perception of PSY1 depends on At1g72300, which is a leucine-rich repeat receptor kinase (LRR-RK) whose two paralogs are involved in the perception of phytosulfokine (PSK), which is a 5-aa tyrosine-sulfated peptide that primarily promotes cellular proliferation. Multiple loss-of-function mutations in these three paralogous LRR-RKs significantly enhanced phenotypes, compared with single disruptants, suggesting that these LRR-RKs have overlapping functions. Triple mutations in these LRR-RKs resulted in dwarfism because of decreases in cell number and cell size and caused insufficiency in tissue repair after wounding. The present results suggest that this paralogous LRR-RK family integrates growth-promoting signals mediated by two structurally distinct sulfated peptides: PSY1 and PSK.