Early events during avian skin appendage regeneration: Dependence on epithelial-mesenchymal interaction and order of molecular reappearance
Early events during avian skin appendage regeneration: Dependence on epithelial-mesenchymal interaction and order of molecular reappearance
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DOI：
10.1111/1523-1747.ep12584254
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发表时间：
1996-10-01
影响因子：
6.5
通讯作者：
Jiang, TX
中科院分区：
文献类型：
--
作者：
Chuong, CM;Widelitz, RB;Jiang, TX
利用上皮-间充质重组培养的鸡皮肤外植体研究了皮肤附件发育和再生过程中的早期分子事件。将外植体上皮与间充质分离，旋转90度或180度，与间充质重组，并培养。在该过程之后，现有的羽芽消失，并再生新的芽。新芽的位置由原始真皮凝聚决定，而方向由原始上皮决定。检查关键形态发生分子的时间表达3，重组后6、20 h进行原位杂交和免疫组化染色。结果表明，（i）基板形成和基板上皮中无翅int（Wnt）7a和Msx-1的表达是间充质依赖的，（ii）前间充质中Hox C6和神经细胞粘附分子（NCAM）的表达是基板上皮依赖的，（iii）骨形态发生蛋白（BMP）-2，BMP-4，并且成纤维细胞生长因子（FGF）-4在原始真皮浓缩物中的表达不受重组的影响。（iv）旧真皮浓缩物可以诱导具有新Wnt 7a、音刺猬（Shh）和Msx-1和Msx-2表达的新基板，（v）新的基板上皮然后可以在羽芽间充质中诱导新的Hox C6和NCAM微梯度。（vi）出现的顺序可以按以下顺序分为四组：BMP-2、BMP-4和FGF-4（肽生长因子）; Wnt 7a和Shh（果蝇节段极性基因同源物）; Msx-1和Msx-2（Msx类同源框基因）;然后是Hox C6（Hox类同源框基因）和NCAM（粘附分子）。这些结果表明，在皮肤附件发育的诱导阶段的分子级联的顺序。
Early molecular events during the development and regeneration of skin appendages were studied using cultured chicken skin explants with epithelial-mesenchymal recombination. The explant epithelium was separated from the mesenchyme, rotated 90 degrees or 180 degrees, recombined with the mesenchyme, and cultured, After this procedure, existing feather buds disappeared and new buds were regenerated, The location of the new buds is determined by the original dermal condensations, whereas the orientation is dictated by the original epithelium, The temporal expression of key morphogenetic molecules was examined 3, 6, and 20 h after recombination by wholemount in situ hybridization and immunostaining. The results showed the following, (i) Placode formation and the expression of wingless-int (Wnt) 7a and Msx-1 in the placode epithelium are mesenchyme dependent, (ii) Hox C6 and neural cell adhesion molecule (NCAM) expression in the anterior mesenchyme is placode epithelium dependent, (iii) Bone morphogenetic protein (BMP)-2, BMP-4, and fibroblast growth factor (FGF)-4 expression in the original dermal condensations was unaffected by recombination, (iv) Old dermal condensations can induce new placodes with new Wnt 7a, sonic hedgehog (Shh), and Msx-1 and -2 expression, (v) The new placode epithelium can then induce new Hox C6 and NCAM microgradients in the feather bud mesenchyme, (vi) The order of appearance can be classified into four groups in the following order: BMP-2, BMP-4, and FGF-4 (peptide growth factors); Wnt 7a and Shh (Drosophila segment polarity gene homologs); Msx-1 and Msx-2 (Msx class homeobox genes); and then Hox C6 (Hox class homeobox genes) and NCAM (adhesion molecules). These results suggest an order for the molecular cascade during the inductive phase of skin appendage development.