An Improved Preparation of Sodium Desoxyribonucleate
An Improved Preparation of Sodium Desoxyribonucleate
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脱氧核糖核酸钠制备方法的改进
DOI：
10.1021/ja01127a034
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发表时间：
1952
影响因子：
15
通讯作者：
A. L. Dounce
中科院分区：
文献类型：
--
作者：
E. Kay;N. S. Simmons;A. L. Dounce
以十二烷基硫酸钠溶液为提取脱蛋白剂，改进了从小牛胸腺中提取脱氧核糖核酸钠的方法。该产品没有对蛋白质进行缩二脲试验，尽管粘度研究表明可能存在后一种试剂无法检测到的微量蛋白质。粘度测定表明，该产品的聚合度很高。紫外吸收曲线的GB*%m 192为260微米，未显示出可检测到的杂质。用凯氏定氮法测定的产品氮含量约为13.5%，用比色法测定的磷含量约为9.3%。纯化产物的产率非常令人满意，每15克胸腺(干重)约为1.5克脱氧核糖核酸钠(干重)。近年来，人们首先通过Mirsky程序从组织中提取脱氧核糖核糖核酸作为脱氧核糖核组蛋白。2·3用乙醇从强盐溶液中沉淀，即可将核酸分离为钠盐。Sevag等人的方法是
An improved preparation of sodium desoxyribonucleate from calf thymus is described, using sodium dodecyl sulfate solu-tion as the extracting and deproteinizing agent. The product gives no biuret test for protein, although viscosity studies indi-cate the possible presence of a trace of protein undetectable by the latter reagent. The degree of polymerization of the prod-uct isvery high as shown by viscosity measurements. The ultraviolet absorption curve, with£*% m 192 at 260 µ, does not demonstrate detectable impurities. The percent, nitrogen of the product, determined by the Kjeldahl method, is about 13.5 and the per cent, phosphorus, determined colorimetrically, is about 9.3. The yield of purified product is very satisfac-tory, being about 1.5 g. of sodium desoxyribonucleate (dry weight) per 15 g. of thymus (dry weight).In recent years desoxyribonucleic acid has been extracted from tissue first as desoxyribonucleo-histone, by the procedure of Mirsky. 2· 3 Sub-sequently the nucleic acid can be isolated as the sodi-um salt by precipitation from strong saline solution with alcohol. The method of Sevag, et al.,* is then