New type of tracheal bioartificial organ treated with detergent: Maintaining cartilage viability is necessary for successful immunosuppressant free allotransplantation
New type of tracheal bioartificial organ treated with detergent: Maintaining cartilage viability is necessary for successful immunosuppressant free allotransplantation
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DOI：
10.1097/00002480-200201000-00006
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发表时间：
2002-01-01
期刊：
影响因子：
4.2
通讯作者：
Shimizu, Y
中科院分区：
文献类型：
--
作者：
Liu, Y;Nakamura, T;Shimizu, Y
本研究探讨了在犬中通过洗涤剂处理制备低抗原性同种异体气管移植物的关键因素。第1组（n = 5），在室温下用洗涤剂处理移植物48 h，并用自来水冲洗直至使用。第2组（n = 4）在4 ℃下进行洗涤剂处理48 h，但省略冲洗步骤。第3组（n = 6），移植物在4 ℃下处理48 h，用生理盐水彻底冲洗，并保存在VC中。然后将移植物用于犬的气管替代，而不使用免疫抑制剂。在第1组和第2组中，以及在第3组的6个移植物中的5个中，上皮和混合腺体已从移植物中完全去除。在第1组和第2组中，软骨活力似乎已经丧失，所有动物在38天内死于气道狭窄。在第3组中，软骨细胞是有活力的，所有动物均顺利存活。这些结果表明，维持气管移植物中的软骨活力是成功的无免疫抑制剂同种异体移植所必需的，因此，维持开放的气道。处理温度和充分冲洗是维持移植物软骨活力的关键因素。
The present investigation examined the key factors in the preparation of low-antigenic tracheal allografts by detergent treatment in dogs. In group 1 (n = 5), the grafts were treated by detergent at room temperature for 48 h and rinsed with running water until use. In group 2 (n = 4), detergent treatment was performed at 4degreesC for 48 h, but the rinsing step was omitted. In group 3 (n = 6), the grafts were treated at 4degreesC for 48 h, rinsed thoroughly with physiologic saline, and stored at VC. The grafts were then used for tracheal replacement without immunosuppressant in dogs. The epithelium and mixed glands had been removed completely from the grafts in groups 1 and 2, and in five of the six grafts of group 3. In groups 1 and 2, cartilage viability appeared to have been lost and all animals died of airway stenosis within 38 days. in group 3, the chondrocytes were viable and all animals survived uneventfully. These results suggest that maintaining cartilage viability in a tracheal graft is necessary for successful immunosuppressant-free allotransplantation and, consequently, to maintain an open airway. The treatment temperature and sufficient rinsing are key factors for maintaining cartilage viability of grafts.