Expression Profiles of Pathogenesis-Related Gene, TaLr35PR1, as it Relate to Lr35-Mediated Adult Plant Leaf Rust Resistance
Expression Profiles of Pathogenesis-Related Gene, TaLr35PR1, as it Relate to Lr35-Mediated Adult Plant Leaf Rust Resistance
复制标题
发病机制相关基因 TaLr35PR1 的表达谱与 Lr35 介导的成年植物叶锈病抗性相关
DOI：
10.1007/s11105-016-0992-0
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发表时间：
2016-12-01
影响因子：
2.1
通讯作者：
Liu, Daqun
中科院分区：
文献类型：
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作者：
Li, Xiaoying;Zhang, Yanjun;Liu, Daqun
植物已经发展出复杂的机制来对抗病原体感染。在应对病原体攻击的获得性模式之一是产生致病相关（PR）蛋白。我们的前期研究报道了从小麦近等基因系TcLr35中克隆到一个编码保守丝氨酸羧肽酶（SCP）结构域蛋白的PR1基因TaLr35PR1。然而，TaLr35PR1在小麦生长和lr35介导的小麦成体对小麦锈病抗性中的作用尚不清楚。本研究发现，在含有Lr35的小麦植株（TcLr35）中，TaLr35PR1受到小麦黑麦草的强烈诱导，TaLr35PR1的表达量显著增加，在12 hpi时达到最大值。TcLr35小麦的TaLr35PR1积累量在不同生长发育时期呈稳定上升趋势，在黑麦黑麦侵染下达到显著峰值，而在模拟接种时则保持相近水平，变化不大。Western blotting证实，接种黑麦小麦后，TaLr35PR1蛋白在TcLr35成株中积累增多，并在接种后120 ~ 168 h保持相近水平。与TaLr35PR1在RNA水平上的表达模式相似，TaLr35PR1蛋白的积累在苗期较弱，成熟期达到峰值并保持不变，这与Lr35基因作为成体植物抗性基因的表达特征一致。这些结果表明，TaLr35PR1参与了小麦生长和lr35介导的小麦对叶锈病病原的防御反应。
Plants have developed sophisticated mechanisms to combat pathogen infection. One of the acquired modes in response to pathogen attack is the production of the pathogenesis-related (PR) proteins. Our earlier studies reported that TaLr35PR1, a PR1 gene encoding a protein with conserved serine carboxypeptidase (SCP) domain, has been cloned from wheat near-isogenic line TcLr35. However, the involvement of TaLr35PR1 in wheat growth and Lr35-mediated adult resistance to Puccinia triticina remains unclear. Here, we showed that TaLr35PR1 was strongly induced by P. triticina in wheat plant containing Lr35 (TcLr35), in which the expression level of TaLr35PR1 significantly increased and reached the maximum at 12 hpi. The accumulations of TaLr35PR1 increased stably and showed significant peak challenged by P. triticina at different growth and development periods of TcLr35 wheat while it maintained similar level and changed little in mock inoculated. Western blotting was conducted to confirm that TaLr35PR1 protein was increasingly accumulated in the TcLr35 adult plants after P. triticina inoculation and maintained at a similar level from 120 to 168 h post-inoculation. Similar to the expression patterns of TaLr35PR1 at RNA levels, the accumulations of TaLr35PR1 protein were weak in the seedling stage and then increased to the peak and kept constant levels at the mature stage which is consistent with the expression feature of Lr35 gene as an adult plant resistance gene. All these findings suggest that TaLr35PR1 is involved in wheat growth and Lr35-mediated adult wheat defense response to leaf rust pathogen attack.