β-Glucans are involved in immune-modulation of THP-1 macrophages
β-Glucans are involved in immune-modulation of THP-1 macrophages
复制标题
DOI：
10.1002/mnfr.201100715
复制
发表时间：
2012-05-01
影响因子：
5.2
通讯作者：
Wichers, Harry J.
中科院分区：
文献类型：
--
作者：
Chanput, Wasaporn;Reitsma, Marit;Wichers, Harry J.
我们旨在研究从不同食物来源（燕麦、大麦和香菇）中提取的β -葡聚糖对肉豆酸盐phorbol acetate （PMA）分化的THP-1巨噬细胞的不同免疫学方面的影响。采用商业纯化的大麦-葡聚糖（商业BG）和香菇多糖来比较相同来源但不同纯度和加工程度的-葡聚糖。方法与结果测定了β -葡聚糖样品的化学成分和分子量分布。炎症相关基因表达动力学(IL-1 β、IL-8、核因子κ B [NF-？100 μ g/mL β -葡聚糖刺激3、6和24 h后的IL-10的变化。所有测试的β -葡聚糖轻度上调观察到的炎症相关基因的差异基因表达模式。β -葡聚糖粗提取物及其相应的商业形式的基因表达动力学相似，但诱导倍数不同。与脂多糖（LPS）处理相比，在暴露于脂多糖（LPS）之前，将THP-1巨噬细胞与β -葡聚糖预孵育可减少炎症相关基因的诱导。β -葡聚糖刺激的THP-1巨噬细胞没有产生一氧化氮（No）和过氧化氢（H2O2）。经葡聚糖刺激后，细胞吞噬活性无明显差异。结论体外分析表明，所分析的β -葡聚糖具有不同程度的免疫调节特性，这可能与β -葡聚糖的结构、分子量和组成特性有关。
Scope We aimed to examine different immunological aspects of beta-glucans derived from different food sources (oat, barley and shiitake) on phorbol myristate acetate (PMA)-differentiated THP-1 macrophages. Commercially purified barley beta-glucan (commercial BG) and lentinan were included to compare beta-glucans from the same origin but different degree of purity and processing. Methods and results Chemical composition and molecular weight distribution of beta-glucan samples were determined. Inflammation-related gene expression kinetics (IL-1 beta, IL-8, nuclear factor kappa B [NF-?B] and IL-10) after 3, 6 and 24 h of stimulation with 100 mu g/mL beta-glucan were investigated. All tested beta-glucans mildly upregulated the observed inflammation-related genes with differential gene expression patterns. Similar gene expression kinetics, but different fold induction values, was found for the crude beta-glucan extracts and their corresponding commercial forms. Pre-incubation of THP-1 macrophages with beta-glucans prior to lipopolysaccharide (LPS) exposure decreased the induction of inflammation-related genes compared to LPS treatment. No production of nitric oxide (NO) and hydrogen peroxide (H2O2) was detected in beta-glucan stimulated THP-1 macrophages. Phagocytic activity was not different after stimulation by beta-glucan samples. Conclusion Based on these in vitro analyses, it can be concluded that the analysed beta-glucans have varying levels of immunomodulating properties, which are likely related to structure, molecular weight and compositional characteristic of beta-glucan.