Diesel Exhaust Induces Systemic Lipid Peroxidation and Development of Dysfunctional Pro-Oxidant and Pro-Inflammatory High-Density Lipoprotein
Diesel Exhaust Induces Systemic Lipid Peroxidation and Development of Dysfunctional Pro-Oxidant and Pro-Inflammatory High-Density Lipoprotein
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DOI：
10.1161/atvbaha.112.300552
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发表时间：
2013-06-01
影响因子：
8.7
通讯作者：
Araujo, Jesus A.
中科院分区：
文献类型：
--
作者：
Yin, Fen;Lawal, Akeem;Araujo, Jesus A.
方法和结果-我们将载脂蛋白E缺陷小鼠暴露于约250 μ g/m3的柴油废气（DE）中2周，暴露于过滤空气（FA）中2周，或暴露于DE中2周，随后FA治疗1周（DE+FA）。DE导致支气管肺泡灌洗液中脂质过氧化作用增强，并伴有对HDL功能的影响。HDL的抗氧化能力通过一种测定法来评估，该测定法通过2 '，7'-二氯荧光素荧光来评估HDL抑制低密度脂蛋白氧化的能力。DE暴露小鼠的HDL显示出23053 +/- 2844相对荧光单位，高于FA暴露小鼠（10282 +/- 1135相对荧光单位，P
Objective-To evaluate whether exposure to air pollutants induces oxidative modifications of plasma lipoproteins, resulting in alteration of the protective capacities of high-density lipoproteins (HDLs).Approach and Results-We exposed apolipoprotein E-deficient mice to diesel exhaust (DE) at approximate to 250 mu g/m(3) for 2 weeks, filtered air (FA) for 2 weeks, or DE for 2 weeks, followed by FA for 1 week (DE+FA). DE led to enhanced lipid peroxidation in the brochoalveolar lavage fluid that was accompanied by effects on HDL functionality. HDL antioxidant capacity was assessed by an assay that evaluated the ability of HDL to inhibit low-density lipoprotein oxidation estimated by 2',7'-dichlorofluorescein fluorescence. HDL from DE-exposed mice exhibited 23053 +/- 2844 relative fluorescence units, higher than FA-exposed mice (10282 +/- 1135 relative fluorescence units, P