Multiplexed fluorescence lifetime measurements by frequency-sweeping Fourier spectroscopy.
Multiplexed fluorescence lifetime measurements by frequency-sweeping Fourier spectroscopy.
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DOI：
10.1364/ol.35.002910
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发表时间：
2010-09-01
期刊：
影响因子：
3.6
通讯作者：
Peng L
中科院分区：
文献类型：
--
作者：
Zhao M;Peng L
我们报告的同时测量荧光寿命在多个激发波长的傅里叶变换频域荧光寿命光谱仪。光谱仪使用迈克尔逊干涉仪，其差分光程长度以22，000Hz扫描速率扫描。光学延迟的扫描速度在每次扫描期间呈线性变化，并产生在45.5 μs内从−150 MHz扫描到150 MHz的干涉调制。频率扫描调制允许纳秒荧光寿命测量在45.5微秒内。由于干涉调制频率与波长相关，因此在傅立叶复用原理下，光谱仪可以同时对多个激发波长进行寿命测量。
We report simultaneous measurements of fluorescence lifetimes at multiple excitation wavelengths with a Fourier transform frequency domain fluorescence lifetime spectrometer. The spectrometer uses a Michelson interferometer with its differential optical path length scanning at 22,000 Hz scan rate. The scan speed of the optical delay varies linearly during each scan and creates interference modulations that sweep from −150 to 150 MHz in 45.5 μs. The frequency-sweeping modulation allows nanosecond fluorescence lifetime measurements within 45.5 microseconds. Because the interference modulation frequency is wavelength-dependent, under the Fourier multiplexing principle, the spectrometer can perform lifetime measurements on multiple excitation wavelengths simultaneously.