The calculation of partial specific volumes of proteins in guanidine hydrochloride.
The calculation of partial specific volumes of proteins in guanidine hydrochloride.
复制标题
盐酸胍中蛋白质部分比容的计算。
DOI：
10.1016/0003-9861(74)90164-7
复制
发表时间：
1974
影响因子：
3.9
通讯作者：
S. N. Timasheff
中科院分区：
文献类型：
--
作者：
James C. Lee;S. N. Timasheff
提出了计算6m盐酸胍溶液中与蛋白质结合的盐酸胍和水分子数的方法。通过这些量的正确组合，可以计算出溶剂组分与蛋白质的优先相互作用参数，进而可以准确预测6m盐酸胍中蛋白质的表观部分比容值。该方法仅需要了解蛋白质的氨基酸组成。将此方法应用于文献数据表明，蛋白质部分比容的预测值使得可以通过 6m 盐酸胍的沉降平衡实验准确估计其亚基分子量。
A method is presented for calculating the number of guanidine hydrochloride and water molecules bound to proteins in 6mguanidine hydrochloride solution. By correct combination of these quantities, the preferential interaction parameter of solvent components with proteins can be calculated, and, in turn, accurate values of the apparent partial specific volumes of proteins in 6mguanidine hydrochloride can be predicted. This method requires only the knowledge of the amino acid composition of the protein. Application of this method to literature data shows that the predicted values of the partial specific volumes of proteins render possible an accurate estimation of their subunit molecular weights from sedimentation equilibrium experiments in 6mguanidine hydrochloride.