Genetic interactions affecting touch sensitivity in Caenorhabditis elegans
Genetic interactions affecting touch sensitivity in Caenorhabditis elegans
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DOI：
10.1073/pnas.93.13.6577
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发表时间：
1996-06-25
影响因子：
11.1
通讯作者：
Chalfie, M
中科院分区：
文献类型：
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作者：
Gu, GQ;Caldwell, GA;Chalfie, M
线虫6个触摸受体神经元对轻触的反应至少需要13个基因（mec-1、mec-2、mec-4-10、mec-22、mec-14、mec-15和mec-18）。其中一些基因的一些隐性等位基因作为mec-1、mec-5、mec-6、mec-12和mec-15的温度敏感等位基因的显性增强突变。对Mec -4和Mec -5显性增强子的筛选产生了先前已知基因的突变，此外，一些Mec -7等位基因显示出对Mec -15触摸不敏感（Mec）表型的等位基因特异性显性抑制。这些突变所表现出的显性增强和抑制表明，几个触摸基因的产物相互作用，这些结果与一个模型一致，由这些基因的已知序列支持，几乎所有的触摸功能基因都参与机械感觉装置。
At least 13 genes (mec-1, mec-2, mec-4-10, mec-22, mec-14, mec-15, and mec-18) are needed for the response to gentle touch by 6 touch receptor neurons in the nematode Caenorhabditis elegans. Several, otherwise recessive alleles of some of these genes act as dominant enhancer mutations of temperature-sensitive alleles of mec-1, mec-5, mec-6, mec-12, and mec-15. Screens for additional dominant enhancers of mec-4 and mec-5 yielded mutations in previously known genes, In addition, some mec-7 alleles showed allele-specific, dominant suppression of the mec-15 touch-insensitive (Mec) phenotype. The dominant enhancement and suppression exhibited by these mutations suggest that the products of several touch genes interact, These results are consistent with a model, supported by the known sequences of these genes, that almost all of the touch function genes contribute to the mechanosensory apparatus.