Immobilized metal ion affinity-based fluorescence polarization (IMAP): Advances in kinase screening
Immobilized metal ion affinity-based fluorescence polarization (IMAP): Advances in kinase screening
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DOI：
10.1089/154065804323056549
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发表时间：
2004-04-01
影响因子：
1.8
通讯作者：
Boge, A
中科院分区：
文献类型：
--
作者：
Sportsman, JR;Gaudet, EA;Boge, A
IMAP(R) 荧光偏振技术是一种用于分析激酶、磷酸酶和磷酸二酯酶的均质无抗体方法。该技术的最新发展包括试剂系统（渐进式结合系统）的增强，显着扩大了 ATP 可用浓度范围、底物选择和测定配置。借助新的渐进式系统，我们能够设计多重检测，从而同时测定多种激酶活性。此外，现在可以进行偶联测定，允许通过激酶级联的自然或人工偶联来测定激酶。
The IMAP(R) Fluorescence Polarization technology is a homogeneous antibody-free method for analysis of kinases, phosphatases, and phosphodiesterases. Recent developments to the technology include an enhancement of the reagent system (the Progressive Binding System) that significantly expands the range of useable concentrations of ATP, choices of substrates, and assay configurations. With the new Progressive System, we are able to design Multiplexed assays that allow the simultaneous determination of multiple kinase activities. In addition, Coupled assays are now possible, allowing the assay of kinases through natural or artificial coupling through kinase cascades.