Functional Phosphoproteomic Analysis Reveals That a Serine-62-Phosphorylated Isoform of Ethylene Response Factor110 Is Involved in Arabidopsis Bolting1[C][W][OA]
Functional Phosphoproteomic Analysis Reveals That a Serine-62-Phosphorylated Isoform of Ethylene Response Factor110 Is Involved in Arabidopsis Bolting1[C][W][OA]
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DOI：
10.1104/pp.112.204487
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发表时间：
2012-11
期刊：
影响因子：
7.4
通讯作者：
Lin Zhu;Dandan Liu;Yaojun Li;Ning Li
中科院分区：
文献类型：
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作者：
Lin Zhu;Dandan Liu;Yaojun Li;Ning Li
简单气体植物激素乙烯通过阴阳信号转导途径调控拟南芥开花。乙烯是一种主要的植物激素，在调节抽薹中起着重要作用，尽管其潜在的分子机制还不清楚。在这项研究中，我们报告了新的发现，丝氨酸-62（Ser-62）的乙烯反应因子110（ERF 110）的磷酸化参与调节抽薹时间。利用拟南芥（Arabidopsis thaliana）的乙烯应答突变体和ERF 110 RNA干扰敲除株系，分析了ERF 110的基因表达和翻译后修饰（磷酸化）。生理生化研究表明，ERF 110的Ser-62磷酸化与抽薹时间密切相关，即乙烯增强了拟南芥ERF 110基因的表达，降低了ERF 110蛋白的Ser-62磷酸化。开花同源异型APETALA 1基因的表达上调的Ser-62-磷酸化亚型的ERF 110转录因子，这是必要的，但不足以正常抽薹。ERF 110基因的表达和磷酸化受乙烯的调控，其调控途径既有乙烯不敏感2依赖的途径，也有乙烯不敏感2非依赖的途径，这构成了乙烯在拟南芥抽薹调控中的一种双重和相反的作用机制。
Summary: The simple gaseous plant hormone ethylene regulates Arabidopsis flowering via Yin-and-Yang signal transduction pathways. Ethylene is a major plant hormone that plays an important role in regulating bolting, although the underlying molecular mechanism is not well understood. In this study, we report the novel finding that the serine-62 (Ser-62) phosphorylation of Ethylene Response Factor110 (ERF110) is involved in the regulation of bolting time. The gene expression and posttranslational modification (phosphorylation) of ERF110 were analyzed among ethylene-response mutants and ERF110 RNA-interfering knockout lines of Arabidopsis (Arabidopsis thaliana). Physiological and biochemical studies revealed that the Ser-62 phosphorylation of ERF110 was closely related to bolting time, that is, the ethylene-enhanced gene expression of ERF110 and the decreased Ser-62 phosphorylation of the ERF110 protein in Arabidopsis. The expression of a flowering homeotic APETALA1 gene was up-regulated by the Ser-62-phosphorylated isoform of the ERF110 transcription factor, which was necessary but not sufficient for normal bolting. The gene expression and phosphorylation of ERF110 were regulated by ethylene via both Ethylene-Insensitive2-dependent and -independent pathways, which constitute a dual-and-opposing mechanism of action for ethylene in the regulation of Arabidopsis bolting.