Association between Polycystic Ovary Syndrome and Gut Microbiota.
Association between Polycystic Ovary Syndrome and Gut Microbiota.
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多囊卵巢综合症与肠道菌群之间的关联
DOI：
10.1371/journal.pone.0153196
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Tang L
中科院分区：
文献类型：
--
作者：
Guo Y;Qi Y;Yang X;Zhao L;Wen S;Liu Y;Tang L
多囊卵巢综合征（PCOS）是育龄妇女最常见的内分泌疾病。多囊卵巢综合征的病因和发病机制复杂，治疗困难。在此，我们描述了肠道微生物群在来曲唑（一种非甾体芳香化酶抑制剂）诱导的PCOS大鼠模型的发病机制和治疗中的作用。通过PCR-DGGE和实时荧光定量PCR检测发情周期、激素水平、卵巢形态和肠道菌群的变化。结果表明，PCOS大鼠表现出异常的动情周期，雄激素生物合成增加，卵巢组织中出现多个大囊肿，颗粒层减少。同时，来曲唑治疗组大鼠的肠道菌群组成与对照组不同。PCOS组乳杆菌、瘤胃球菌和梭菌的检出率低于对照组，而普雷沃菌的检出率高于对照组。用乳酸杆菌和来自健康大鼠的粪便微生物群移植（FMT）治疗PCOS大鼠后，发现FMT组8只大鼠的发情周期均得到改善，乳酸杆菌移植组8只大鼠中有6只大鼠的发情周期得到改善，雄激素生物合成减少。他们的卵巢形态正常化。FMT组和乳酸菌组肠道菌群组成均有所恢复，乳酸菌和梭菌增加，普雷沃菌减少。这些结果表明，肠道菌群失调与PCOS的发病机制有关。通过FMT和乳酸杆菌移植进行微生物群干预有利于PCOS大鼠的治疗。
Polycystic ovary syndrome (PCOS) is the most frequent endocrinopathy in women of reproductive age. It is difficult to treat PCOS because of its complex etiology and pathogenesis. Here, we characterized the roles of gut microbiota on the pathogenesis and treatments in letrozole (a nonsteroidal aromatase inhibitor) induced PCOS rat model. Changes in estrous cycles, hormonal levels, ovarian morphology and gut microbiota by PCR-DGGE and real-time PCR were determined. The results showed that PCOS rats displayed abnormal estrous cycles with increasing androgen biosynthesis and exhibited multiple large cysts with diminished granulosa layers in ovarian tissues. Meanwhile, the composition of gut microbiota in letrozole-treated rats was different from that in the controls. Lactobacillus, Ruminococcus and Clostridium were lower while Prevotella was higher in PCOS rats when compared with control rats. After treating PCOS rats with Lactobacillus and fecal microbiota transplantation (FMT) from healthy rats, it was found that the estrous cycles were improved in all 8 rats in FMT group, and in 6 of the 8 rats in Lactobacillus transplantation group with decreasing androgen biosynthesis. Their ovarian morphologies normalized. The composition of gut microbiota restored in both FMT and Lactobacillus treated groups with increasing of Lactobacillus and Clostridium, and decreasing of Prevotella. These results indicated that dysbiosis of gut microbiota was associated with the pathogenesis of PCOS. Microbiota interventions through FMT and Lactobacillus transplantation were beneficial for the treatments of PCOS rats.