Study of thiazole orange in aptamer-based dye-displacement assays
Study of thiazole orange in aptamer-based dye-displacement assays
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DOI：
10.1007/s00216-007-1773-2
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发表时间：
2008-02-01
影响因子：
4.3
通讯作者：
Stojanovic, Milan N.
中科院分区：
文献类型：
--
作者：
Pei, Renjun;Stojanovic, Milan N.
我们筛选了一系列RNA和DNA适体，以确定它们在染料置换分析中与to染料竞争的能力。我们的结论是，虽然TO染料置换方法的性能并不总是可预测的，但它仍然是一种简单而敏感的检测RNA适体与小分子之间结合的方法。特别地，我们描述了妥布霉素和茶碱的有效测定，观察到高达90%的to置换，我们描述了第一个cAMP的适配体测定。
We screened a series of RNA and DNA aptamers for their ability to serve in the dye displacement assays in which analytes compete with TO dye. We conclude that, while the performance of the TO dye displacement approach is not always predictable, it is still a simple and sensitive assay to detect binding between RNA aptamers and small molecules. In particular, we describe efficient assays for tobramycin and theophylline, with up to 90% displacement of TO observed, and we describe the first aptameric assay for cAMP.