TISSUE PLASMINOGEN-ACTIVATOR MESSENGER-RNA IN MURINE TISSUES
TISSUE PLASMINOGEN-ACTIVATOR MESSENGER-RNA IN MURINE TISSUES
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DOI：
10.1016/0014-5793(88)80806-8
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发表时间：
1988-02-29
期刊：
影响因子：
3.5
通讯作者：
STRICKLAND, S
中科院分区：
文献类型：
--
作者：
RICKLES, RJ;STRICKLAND, S
尿激酶型和组织型纤溶酶原激活剂是在哺乳动物中发现的两种酶，它们特异性地将酶原纤溶酶原转化为纤溶酶。使用 cDNA 探针，我们检测了小鼠组织中两种类型的纤溶酶原激活剂 mRNA 的存在。我们证明组织型纤溶酶原激活剂 mRNA 可以在多种组织中检测到。相比之下，仅在少数分析的组织中观察到尿激酶型纤溶酶原激活剂 mRNA 的积累。使用 S1 核酸酶测定，我们证明检测到的 tPA mRNA 包含编码非蛋白酶指、生长因子和 kringle 结构域的完整序列。
The urokinase‐type and tissue‐type plasminogen activators are the two enzymes found in mammals, which specifically convert the zymogen plasminogen to plasmin. Using cDNA probes, we have assayed for the presence of the two types of plasminogen activator mRNAs in murine tissues. We demonstrate that tissue‐type plasminogen activator mRNA can be detected in a wide variety of tissues. In contrast, the accumulation of urokinase‐type plasminogen activator mRNA is observed in only a few of the tissues analyzed. Using an S1nuclease assay, we demonstrate that the tPA mRNA detected contains the complete sequences encoding the non‐protease finger, growth‐factor and kringle domains.