Projector:: automatic contig mapping for gap closure purposes -: art. no. e144
Projector:: automatic contig mapping for gap closure purposes -: art. no. e144
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DOI：
10.1093/nar/gng144
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发表时间：
2003-11-15
影响因子：
14.9
通讯作者：
Kok, J
中科院分区：
文献类型：
--
作者：
van Hijum, SAFT;Zomer, AL;Kok, J
Projector 设计用于将未完成的原核基因组中的重叠群自动定位到密切相关的菌株或物种的模板基因组上。投影仪将乳酸乳球菌 MG1363 的 84 个重叠群（对应于 81% 的组装核苷酸）与乳酸乳球菌 IL1403 的基因组进行映射。 93% 的后续缺口闭合 PCR 成功。此外，使用投影仪后，观察到 N50 和 N80 值（描述装配质量）显着改善。由于正在对越来越多的细菌基因组进行测序，Projector 提供了一种有效的方法来弥补基因组测序项目后期的大量剩余空白。
Projector was designed for automatic positioning of contigs from an unfinished prokaryotic genome onto a template genome of a closely related strain or species. Projector mapped 84 contigs of Lactococcus lactis MG1363 (corresponding to 81% of the assembly nucleotides) against the genome of L.lactis IL1403. Ninety three percent of subsequent gap closure PCRs were successful. Moreover, a significant improvement in the N50 and N80 values (describing the assembly quality) was observed after the use of Projector. Because increasing numbers of bacterial genomes are being sequenced, Projector provides an efficient method to close a significant number of remaining gaps in the late stages of a genome sequencing project.