Pulmonary Toxicity of Well-dispersed Single-wall Carbon Nanotubes Following Intratracheal Instillation
Pulmonary Toxicity of Well-dispersed Single-wall Carbon Nanotubes Following Intratracheal Instillation
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DOI：
10.4028/www.scientific.net/jnanor.18-19.9
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发表时间：
2012-01-01
影响因子：
1.7
通讯作者：
Tanaka, Isamu
中科院分区：
文献类型：
--
作者：
Morimoto, Yasuo;Hirohashi, Masami;Tanaka, Isamu
利用超声波法将单壁碳纳米管（SWCNTs）均匀分散，并进行气管内滴注研究。在含有0.1%Triton X-100的蒸馏水中，SWCNT的几何平均直径和长度分别为44 nm和0.69 μ m。大鼠气管内接受0.2 mg或0.4 mg SWCNT，对照组接受含有0.1% Triton X-100的蒸馏水的气管内滴注。分别于给药后3天、1周、1个月、3个月和6个月处死大鼠。支气管肺泡灌洗液（BALF）和病理学特征显示，SWCNT剂量诱导大鼠肺中持续的中性粒细胞浸润。在苦参碱诱导的中性粒细胞趋化因子（CINC）家族中，SWCNT暴露组BALF中CINC-1和CINC-2的浓度持续增加。BALF中HO-1的浓度也持续上调。这些数据表明，在本研究中，分散良好的SWCNT具有致炎潜力。
Single-wall carbon nanotubes (SWCNTs) were well-dispersed using ultrasonication to conduct an intratracheal instillation study. The geometric mean diameter and length of the SWCNT in distilled water including 0.1 % Triton X-100 was 44 nm and 0.69 mu m, respectively. Rats intratracheally received 0.2 mg or 0.4 mg of SWCNT, and a control group received intratracheal instillation of distilled water containing 0.1 % Triton X-100. The rats were then sacrificed at 3 days, 1 week, 1 month, 3 months and 6 months after instillation. Bronchoalveolar lavage fluid (BALF) and pathological features revealed that the dose of SWCNT induced persistent neutrophil infiltration in rat lungs. In the cytokine-induced neutrophil chemoattractants (CINCs) family, the concentrations of CINC-1 and CINC-2 in the BALF increased persistently in the SWCNT-exposed groups. The concentration of HO-1 in the BALF was also up-regulated persistently in the exposed groups. These data suggested that well-dispersed SWCNT had an inflammatory potential in the present study.