SYNTHESIS OF PHOSPHATIDYLCHOLINE AND PHOSPHATIDYLETHANOLAMINE AT DIFFERENT AGES IN THE RAT-BRAIN INVITRO
SYNTHESIS OF PHOSPHATIDYLCHOLINE AND PHOSPHATIDYLETHANOLAMINE AT DIFFERENT AGES IN THE RAT-BRAIN INVITRO
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DOI：
10.1007/bf02533507
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发表时间：
1979-01-01
期刊：
影响因子：
1.9
通讯作者：
PORCELLATI, G
中科院分区：
文献类型：
--
作者：
BRUNETTI, M;GAITI, A;PORCELLATI, G
脑微粒体18mo胆碱和乙醇胺磷酸甘油酯的从头合成以标记胞苷-5‘’-二磷酸胆碱和胞苷-5‘’-二磷酸乙醇胺为脂质前体，对老年雄性大鼠进行了体外研究。与成年动物相比，这两类磷脂的合成速率明显降低。将外源性甘油二酯添加到衰老大鼠脑微粒体中，使其合成速度接近成人水平。衰老大鼠肝微粒体中胆碱和乙醇胺磷酸甘油酯的合成不受影响。衰老大鼠脑微粒体中脂肪酸的摩尔分布与成年动物有显著差异。单烯和双烯物种的含量增加，而四烯物种的含量减少。胆碱和乙醇胺掺入各自的磷脂中的碱基交换反应在老年大鼠脑微粒体中不受影响。
The de novo synthesis of choline and ethanolamine phosphoglycerides in brain microsomes from 18 mo. old male rats was investigated in vitro by using labeled cytidine-5''-diphosphate choline and cytidine-5''-diphosphate ethanolamine as lipid precursors. The rate of synthesis of the 2 phospholipid classes was noticeably decreased, as compared to that of adult animals. The addition of exogenous diacyl glycerols to microsomes from aging rat brain brings the rate of synthesis nearly to the adult levels. The synthesis of choline and ethanolamine phosphoglycerides is not affected in the liver microsomes of aging rats. The molar distribution of fatty acids in brain microsomal diacyl glycerols of aging rats is noticeably different from that of adult animals. The content of monoenoic and dienoic species is increased, whereas that of the tetraenoic species is decreased. Base exchange reaction for choline and ethanolamine incorporation into respective phospholipids is not affected in the brain microsomes of the aged rats.