The therapeutic impact of human neonatal BMSC in a right ventricular pressure overload model in mice
The therapeutic impact of human neonatal BMSC in a right ventricular pressure overload model in mice
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人新生儿骨髓间充质干细胞对小鼠右心室压力超负荷模型的治疗作用
DOI：
10.1186/s13287-020-01593-y
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发表时间：
2020-03
影响因子：
7.5
通讯作者：
Chen Huiwen
中科院分区：
文献类型：
--
作者：
Rong Liufu;Shi Guocheng;He Xiaomin;Lv Jingjing;Liu Wei;Zhu Fang;Wen Chen;Zhu Zhongqun;Chen Huiwen
目的探讨供体年龄对骨髓间充质干细胞（BMSCs）治疗右心室压力超负荷所致心室重构的影响。（< 1个月），婴儿（1个月至1岁），幼儿比较两组患者的迁移潜能、表面标志物表达、VEGF分泌和基质金属蛋白（MMP）9表达。对4周龄雄性C57小鼠进行肺动脉结扎，并随机分为治疗组和未治疗对照组。在手术过程中，骨髓间充质干细胞通过心肌内注射到RV游离壁中给予小鼠。四周后，RV功能和组织进行了分析，超声心动图，组织学，定量实时聚合酶链反应。结果人新生儿BMSCs表现出最大的迁移能力和血管内皮生长因子的分泌，但表面标志物的表达没有差异。新生BMSCs给药导致PA结扎后小鼠VEGF表达增加，RV壁厚度和内径显著减少。这些有益的影响可能与旁分泌没有心肌细胞转分化observed.ConclusionsHuman BMSCs从不同年龄组有不同的特点，和最年轻的BMSCs可能有利的影响应用干细胞为基础的治疗，以减轻压力超负荷诱导的RV重构不良。
ObjectiveTo determine the impact of donor age on the therapeutic effect of bone marrow-derived mesenchymal stem cells (BMSCs) in treating adverse remodeling as the result of right ventricle (RV) pressure overload.MethodsBMSCs were isolated from neonatal (< 1 month), infant (1 month to 1 year), and young children (1 year to 5 years) and were compared in their migration potential, surface marker expression, VEGF secretion, and matrix metalloprotein (MMP) 9 expression. Four-week-old male C57 mice underwent pulmonary artery banding and randomized to treatment and untreated control groups. During the surgery, BMSCs were administered to the mice by intramyocardial injection into the RV free wall. Four weeks later, RV function and tissue were analyzed by echocardiography, histology, and quantitative real-time polymerase chain reaction.ResultsHuman neonatal BMSCs demonstrated the greatest migration capacity and secretion of vascular endothelial growth factor but no difference in expression of surface markers. Neonate BMSCs administration resulted in increasing expression of VEGF, a significant reduction in RV wall thickness, and internal diameter in mice after PA banding. These beneficial effects were probably associated with paracrine secretion as no cardiomyocyte transdifferentiation was observed.ConclusionsHuman BMSCs from different age groups have different characteristics, and the youngest BMSCs may favorably impact the application of stem cell-based therapy to alleviate adverse RV remodeling induced by pressure overload.
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影响因子：
5.3
作者：
通讯作者：
--
影响因子：
5.3
作者：
Khan M;Mohsin S;Khan SN;Riazuddin S
通讯作者：
Riazuddin S
DOI：
10.1073/pnas.1712612114
发表时间：
2017-11-07
影响因子：
11.1
作者：
Gesmundo I;Miragoli M;Carullo P;Trovato L;Larcher V;Di Pasquale E;Brancaccio M;Mazzola M;Villanova T;Sorge M;Taliano M;Gallo MP;Alloatti G;Penna C;Hare JM;Ghigo E;Schally AV;Condorelli G;Granata R
通讯作者：
Granata R
影响因子：
37.8
作者：
Hofmann, M;Wollert, KC;Drexler, H
通讯作者：
Drexler, H
影响因子：
8.3
作者：
Izumiya, Y;Shiojima, I;Walsh, K
通讯作者：
Walsh, K