The effect of bile, bile acids and detergents on calcium absorption in the chick.
The effect of bile, bile acids and detergents on calcium absorption in the chick.
复制标题
胆汁、胆汁酸和清洁剂对雏鸡钙吸收的影响。
DOI：
10.1042/bj0970408
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发表时间：
1965
期刊：
影响因子：
--
通讯作者：
E. Holdsworth
中科院分区：
文献类型：
--
作者：
D. D. Webling;E. Holdsworth
1. 通过肠环和口服给药方法进行体内测试，来自佝偻病雏鸡或正常雏鸡的胆汁导致经维生素 D(3) 处理的佝偻病雏鸡肠道对可溶性钙的吸收立即增加。 2. 这种作用显然完全是由于胆汁中存在的牛磺酸结合的胆汁酸，与维生素 D 的作用无关。 3. 鸡胆汁和胆汁酸可以增加以微溶磷酸氢钙形式存在的钙的溶解度和吸收。 4. 此外，至少在某种程度上，胆汁对于雏鸡肠道吸收维生素 D(3) 是必需的，这会间接增强钙的吸收。 5. 因此，胆汁对于小鸡吸收钙具有三重作用。有人认为，对微溶形式的钙的直接作用具有相当大的生理重要性，因为正常鸟类饮食中的大部分钙都是这种形式。 6. 胆汁酸增强雏鸡小肠各个区域对钙的吸收。 7. 在经过测试可增强钙吸收的一系列胆汁酸和去污剂中，各种牛磺酸结合胆汁酸和十二烷基硫酸钠（一种阴离子去污剂）是有效的。非离子型去污剂（Tween 80）和阳离子型去污剂（Zephiran）没有效果。 8. 物质直接增加肠道对可溶性钙吸收的能力似乎在某种程度上取决于阴离子去垢作用，即形成在水相和脂相中都具有一定程度可溶性的盐或复合物的能力。 9. 在雏鸡中，骨骼中钙 ((45)Ca) 的直接沉积密切反映了血浆钙放射性的任何增加，无论增加的原因如何，也无论维生素 D(3) 状态如何。虽然十二烷基硫酸钠可以显着增加肠道对钙的吸收并立即沉积在骨骼中，但对佝偻病没有显着效果。 10. 讨论了这些发现的一些影响。
1. Bile from rachitic or normal chicks causes an immediate increase in the intestinal absorption of soluble calcium in rachitic and vitamin D(3)-treated chicks as tested in vivo by intestinal-loop and oral-dosing methods. 2. This effect is apparently solely due to the taurine-conjugated bile acids present in the bile and is independent of the action of vitamin D. 3. Chick bile and bile acids can increase the solubility and the absorption of calcium presented as sparingly soluble calcium hydrogen phosphate. 4. In addition, bile is necessary to some extent at least for the intestinal absorption of vitamin D(3) in the chick and this would indirectly enhance the absorption of calcium. 5. Thus bile is capable of a threefold action in the absorption of calcium in the chick. It is suggested that the direct action on sparingly soluble forms of calcium is of considerable physiological importance since most of the calcium in the normal bird's diet would be in this form. 6. Bile acids enhance the absorption of calcium in all regions of the small intestine of the chick. 7. Of a range of bile acids and detergents tested for enhancement of calcium absorption, various taurine-conjugated bile acids and sodium lauryl sulphate, an anionic detergent, are effective. A non-ionic detergent (Tween 80) and a cationic detergent (Zephiran) are without effect. 8. The ability of a substance to increase directly the intestinal absorption of soluble calcium appears to depend to some extent on an anionic detergent action, i.e. the ability to form a salt or complex soluble to some extent in both aqueous and lipid phases. 9. In chicks the immediate deposition of calcium ((45)Ca) in the bones closely reflects any increase in plasma calcium radioactivity regardless of the cause of the increase and regardless of the vitamin D(3) status. Although sodium lauryl sulphate can increase markedly the calcium absorption from the gut and the immediate deposition in the bones it has no significant effect on rickets. 10. Some of the implications of these findings are discussed.