Natural antimicrobials subtilosin and lauramide arginine ethyl ester synergize with conventional antibiotics clindamycin and metronidazole against biofilms of Gardnerella vaginalis but not against biofilms of healthy vaginal lactobacilli
Natural antimicrobials subtilosin and lauramide arginine ethyl ester synergize with conventional antibiotics clindamycin and metronidazole against biofilms of Gardnerella vaginalis but not against biofilms of healthy vaginal lactobacilli
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DOI：
10.1093/femspd/ftv018
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发表时间：
2015-07-01
影响因子：
3.3
通讯作者：
Chikindas, Michael L.
中科院分区：
文献类型：
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作者：
Algburi, Ammar;Volski, Anna;Chikindas, Michael L.
本研究的目的是评估克林霉素和甲硝唑与天然抗菌剂协同对抗细菌性阴道病（BV）相关的阴道加德纳菌生物膜的能力。确定每种抗菌剂的生物膜细胞（MBC-B）的最低杀菌浓度。月桂酰胺精氨酸乙酯（LAE）、枯草杆菌素、克林霉素和甲硝唑的MBCs-B分别为50、69.5、20和500 μ g/mL。使用棋盘试验和等效线图分析这些抗菌剂之间的相互作用类型。甲硝唑与天然抗菌剂的组合不抑制嗜酸乳酸杆菌。克林霉素与LAE或subtilosin联合使用对乳酸菌有抑制作用，但对生物膜相关的乳酸菌无抑制作用。所有测试的抗微生物组合对BV-相关的移动弯孢菌和厌氧消化链霉菌具有抑制作用。LAE和subtilosin与克林霉素和甲硝唑协同抗革兰氏阴性杆菌生物膜。而不是生物膜相关的阴道乳酸杆菌。BV相关病原体的生物膜可以通过常规抗生素和天然抗菌剂的协同作用组合来控制，这将有助于更好地管理当前的抗生素，特别是考虑到强大的细菌耐药性。我们的发现为有效控制阴道感染的新策略奠定了基础。
The purpose of this study was to evaluate the ability of clindamycin and metronidazole to synergize with natural antimicrobials against biofilms of bacterial vaginosis (BV)-associated Gardnerella vaginalis. Minimum bactericidal concentrations for biofilm cells (MBCs-B) were determined for each antimicrobial. The MBCs-B of lauramide arginine ethyl ester (LAE), subtilosin, clindamycin and metronidazole were 50, 69.5, 20 and 500 mu g mL(-1), respectively. A checkerboard assay and isobologram were used to analyze the type of interactions between these antimicrobials. The combination of metronidazole with natural antimicrobials did not inhibit planktonic lactobacilli. Clindamycin with either LAE or with subtilosin was inhibitory for planktonic but not for biofilm-associated lactobacilli. All tested antimicrobial combinations were inhibitory for BV-associated Mobiluncus curtisii and Peptostreptococcus anaerobius. LAE and subtilosin synergized with clindamycin and metronidazole against biofilms of G. vaginalis but not biofilm-associated vaginal lactobacilli. The biofilms of BV-associated pathogens can be controlled by synergistically acting combinations of conventional antibiotics and natural antimicrobials which will help better management of current antibiotics, especially considering robust bacterial resistance. Our findings create a foundation for a new strategy in the effective control of vaginal infections.