Structural studies of the capsular polysaccharide produced by Leuconostoc mesenteroides ssp. cremoris PIA2.
Structural studies of the capsular polysaccharide produced by Leuconostoc mesenteroides ssp. cremoris PIA2.
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DOI：
10.1021/bm200177z
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发表时间：
2011-05
影响因子：
6.2
通讯作者：
M. Svensson;Xue Zhang;E. Huttunen;G. Widmalm
中科院分区：
文献类型：
--
作者：
M. Svensson;Xue Zhang;E. Huttunen;G. Widmalm
肠膜明串珠菌（Leuconostoc mesenteroides ssp.）使用组分分析和NMR光谱法测定了cremoris PIA 2。(1)H和（13）C共振使用2D NMR实验进行归属，并且通过（1）H，（1）H-NOESY和（1）H，（13）C-HMBC实验获得序列信息。CPS由具有以下结构的线性五糖重复单元组成：→3）-β-D-Galf-（1→6）-β-D-Galf-（1→2）-β-D-Galf-（1→6）-β-D-Galf-（1→3）-β-D-Galp-（1→），其中五个糖残基中的四个具有呋喃环形式，这是在细菌中发现但在哺乳动物中不存在的结构实体。通过对五元糖环异头质子的同调三键耦合常数的大小分析，发现在伯碳原子上取代的糖残基表现出一种构象偏好，而在仲碳原子上取代的糖残基表现出另一种构象偏好.
The structure of the capsular polysaccharide (CPS) produced by Leuconostoc mesenteroides ssp. cremoris PIA2 has been determined using component analysis and NMR spectroscopy. (1)H and (13)C resonances were assigned using 2D NMR experiments, and sequential information was obtained by (1)H,(1)H-NOESY and (1)H,(13)C-HMBC experiments. The CPS consists of linear pentasaccharide repeating units with the following structure: →3)-β-D-Galf-(1→6)-β-D-Galf-(1→2)-β-D-Galf-(1→6)-β-D-Galf-(1→3)-β-D-Galp-(1→, in which four out of the five sugar residues have the furanoid ring form, a structural entity found in bacteria but not in mammals. The analysis of the magnitude of the homonuclear three-bond coupling constants of the anomeric protons for the five-membered sugar rings indicates that the sugar residues substituted at a primary carbon atom show one kind of conformational preferences, whereas those substituted at a secondary carbon atom show another kind of conformational preferences.